Thesis (MSc (Biochemistry))--University of Stellenbosch, 2005. / Endogenous glucocorticoids (GCs) modulate many physiological functions in the human
body and synthetic GCs are the most effective therapy in the treatment of inflammation,
autoimmune and endocrine disorders. However, the long-term usage of synthetic GCs is
associated with severe side-effects. GCs mediate their effects through the ligand-dependent
transcription factor, the glucocorticoid receptor (GR), either by causing an increase
(transactivation) or a decrease (transrepression) in gene transcription. The bioactivity of a
ligand in GR-mediated transcriptional regulation is established by a transcriptional doseresponse
curve, where the potency (EC50 value) and the efficacy (maximal response) of the
ligand are determined. A central question is how different GR ligands elicit their differential
physiological responses for the same gene in the same cell. The main aim of this thesis is to
investigate if the phosphorylation of GR at serine 211 (Ser211) correlates with the potency
and/or efficacy of a particular ligand in transactivation and transrepression of gene expression.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:sun/oai:scholar.sun.ac.za:10019.1/2497 |
| Date | 12 1900 |
| Creators | Stubsrud, Elisabeth |
| Contributors | Hapgood, J. P., Louw, A., Ronacher, K., University of Stellenbosch. Faculty of Science. Dept. of Biochemistry. |
| Publisher | Stellenbosch : University of Stellenbosch |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Format | 1325038 bytes, application/pdf |
| Rights | University of Stellenbosch |
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