Dietary fats have been proposed to alter the amount of glucose transporters in various tissues. This study examined how diets containing linoleic or palmitic fatty acids affected the amount of the major insulin-responsive glucose transporter protein, GLUT-4, in red vastus muscle of mice. At 8 weeks of age, 28 healthy female mice were separated into 3 dietary groups, one control group (5% corn oil fat) and two high fat (15% fat) groups. One of the high fat diets was a linoleic acid rich diet (76% linoleic polyunsaturated fat), while the other was a palmitic acid rich diet (95% palmitic saturated fat). The mice remained on their respective diets for 12-13 weeks until sacrifice. Red vastus muscle samples were removed and prepared for GLUT-4 protein analysis. Homogenized red vastus muscle samples were separated by SDSPAGE, transfered to membrane paper, and immunoblotted. scanning densitometry determined the relative quantity of GLUT-4 from each sample. TAP GLUT-4 protein in the group fed the linoleic acid rich diet was 9% higher than the group fed the low fat diet, and 37% higher than the group fed the palmitic acid rich diet. These data suggest that a prolonged high fat diet consisting of linoleic or palmitic fatty acids play a role in the regulation of GLUT-4 protein content. / School of Physical Education
| Identifer | oai:union.ndltd.org:BSU/oai:cardinalscholar.bsu.edu:handle/184408 |
| Date | January 1992 |
| Creators | Russo, Joseph F. |
| Contributors | Ball State University. School of Physical Education., Mahon, Anthony D. |
| Source Sets | Ball State University |
| Detected Language | English |
| Format | vii, 46 leaves : ill. ; 28 cm. |
| Source | Virtual Press |
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