Flavonoids are a class of plant secondary metabolites that fulfill many functions in planta and also benefit human health. Glucosylation of flavonoids, catalyzed by glucosyltransferases (GTs), help flavonoids perform their important roles. Citrus paradisi (grapefruit) is known to be active in flavonoid metabolism and possesses a variety of secondary product GTs. This research is designed to test the hypothesis that PGT10 is a flavonoid glucosyltransferase. PGT10 was cloned, expressed, purified, and tested for GT activity with a variety of phenolic substrates. Efforts to identify the reaction catalyzed by PGT10 continue. A second project was designed to test the hypothesis that multiple bands obtained by RT-PCR of PGT 5/6 found in root cDNA represented a set of homologous genes. Hypothesis has been confirmed by the homologous fragments found. Additional structural information is presented.
| Identifer | oai:union.ndltd.org:ETSU/oai:dc.etsu.edu:etd-2475 |
| Date | 01 May 2011 |
| Creators | Lin, Zhangfan |
| Publisher | Digital Commons @ East Tennessee State University |
| Source Sets | East Tennessee State University |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | Electronic Theses and Dissertations |
| Rights | Copyright by the authors. |
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