Haemoglobin concentration is becoming a widely popular parameter to use to assess physiological condition within a broad range of species. Assessments of large populations would preferable be done in field to receive quick results and avoid confounding factors associated with transport of blood. A validation study is here performed to see how well the point-of-care device HemoCue Hb 201 + can assess haemoglobin concentration on avian blood. Nucleated erythrocytes have previously been pointed out as something that makes it problematic to apply HemoCue Hb 201 +, designed for human blood, on avian blood. Here it is shown that HemoCue Hb 201 + accurately can estimate haemoglobin concentration for chicken-, tinamou-, and ostrich blood. However, manipulation of ostrich cells, to yield a larger mean corspuscular volume, results in HemoCue Hb 201 + overestimating haemoglobin concentration. A large mean corpuscular volume could therefore be something that impair accuracy in values retrieved with HemoCue Hb 201 +. This study shows that HemoCue Hb 201 + seems possible to apply on avian blood to some extent, but highlights the importance of validation studies when applying this device on new species.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:liu-120106 |
| Date | January 2015 |
| Creators | Gustavsson, Frida |
| Publisher | Linköpings universitet, Biologi, Linköpings universitet, Tekniska fakulteten |
| Source Sets | DiVA Archive at Upsalla University |
| Language | English |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
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