Telomerase, a ribonucleoprotein enzyme has its own internal RNA template that elongates telomeres and stabilizes chromosome structure. The mayority of hepatoma express high levels of human telomerase template RNA ( hTR ) that is essential for cellular telomerase activity. In this study, we examined whether hTR¡Bantisence hTR and IFN£\ gene had a telomerase activity inhibitory effect on J5 hepatoma cell line, through transfection via an expression vector . The expression of mRNAs for hTERT¡BhTR¡Bc-myc¡BIFN£\¡Bp16 and p27 were also detected.
The results suggested that inhibition of telomerase activity and overexpression of hTR gene were observed in FhTR-treated J5 cells. The expression of mRNAs for hTERT¡Bc-myc¡BIFN£\¡Bp16 and p27 genes were not changed whether telomerase was inhibited or not. In addition, genomic DNA fragmentation was observed in telomerase inhibited J5 cells indicating that apoptosis was undergoing in these cells.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0722100-012327 |
| Date | 22 July 2000 |
| Creators | Chao, Shou-Bin |
| Contributors | Jiin-Tsuey Cheng, Chung-Lung Cho, Yi-Ren Hong |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0722100-012327 |
| Rights | campus_withheld, Copyright information available at source archive |
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