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Telomerase, a ribonucleoprotein enzyme has its own internal RNA template that elongates telomeres and stabilizes chromosome structure. The mayority of hepatoma express high levels of human telomerase template RNA ( hTR ) that is essential for cellular telomerase activity. In this study, we examined whether hTR¡Bantisence hTR and IFN£\ gene had a telomerase activity inhibitory effect on J5 hepatoma cell line, through transfection via an expression vector . The expression of mRNAs for hTERT¡BhTR¡Bc-myc¡BIFN£\¡Bp16 and p27 were also detected. The results suggested that inhibition of telomerase activity and overexpression of hTR gene were observed in FhTR-treated J5 cells. The expression of mRNAs for hTERT¡Bc-myc¡BIFN£\¡Bp16 and p27 genes were not changed whether telomerase was inhibited or not. In addition, genomic DNA fragmentation was observed in telomerase inhibited J5 cells indicating that apoptosis was undergoing in these cells.

Thesis (M.S.)--University of Wisconsin--Madison, 1969. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).

This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).

published_or_final_version / Medical Sciences / Master / Master of Medical Sciences

Thesis (M. Med. Sc.)--University of Hong Kong, 2000. / Includes bibliographical references (leaves 30-35).

Thesis (M.Med.Sc.)--University of Hong Kong, 2000. / Includes bibliographical references (leaves 30-35). Also available in print.

A comparison has been made of the formate-C¹⁴ incorporation into the nucleic acid purines and thymine of regenerating rat liver and Novikoff hepatoma in vivo. The effects of these tissues on one another, and on the host tissues has been studied. The utilization of formate by the nucleic acids of Novikoff hepatoma and regenerating rat liver was not significantly altered in animals containing both of these rapidly dividing tissues. The results indicated that the demand for formate by one of the rapidly growing tissues did not lower the uptake of formate by the nucleic acids of the other tissue. Furthermore it was indicated that nucleic acid synthesis in regenerating liver did not alter the synthesis of nucleic acids in other tissues. Regenerating liver and Novikoff hepatoma had no effect on the nucleic acid metabolism of the host tissues of animals bearing one or both of these tissues. These results are not completely in agreement with those reported in the literature. In a preliminary experiment a radioactive suspension of Novikoff hepatoma was transplanted into rats. Twenty percent of the injected radioactivity was recovered in the urine during the first 24 hours of tumor growth. The specific activities of the nucleic acid bases of the tumor, obtained after 24 hours of growth, were negligible. These findings indicated that the nucleic acids of the donor tumor suspension were not utilized in the synthesis of the nucleic acids of the growing tumor. / Medicine, Faculty of / Biochemistry and Molecular Biology, Department of / Graduate

This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).