Understanding the functional impact of physical interactions between proteins and
DNA on gene expression is important for developing approaches to correct disease-associated gene dysregulation. I conducted a systematic, functional genetic analysis of protein-DNA interactions in the promoter region of the yeast ribonucleotide reductase
subunit gene RNR3. I measured the transcriptional impact of systematically
perturbing the major transcriptional regulator, Crt1, and three X-box sites on the
DNA known to physically bind Crt1. This analysis revealed interactions between
two of the three X-boxes in the presence of Crt1, and unexpectedly, a significant
functional role of the X-boxes in the absence of Crt1. Further analysis revealed Crt1-
independent regulators of RNR3 that were impacted by X-box perturbation. Taken
together, these results support the notion that higher-order X-box-mediated interactions
are important for RNR3 transcription, and that the X-boxes have unexpected roles in the regulation of RNR3 transcription that extend beyond their interaction with Crt1.
| Identifer | oai:union.ndltd.org:uottawa.ca/oai:ruor.uottawa.ca:10393/30168 |
| Date | January 2013 |
| Creators | Voll, Sarah |
| Contributors | Kaern, Mads |
| Publisher | Université d'Ottawa / University of Ottawa |
| Source Sets | Université d’Ottawa |
| Language | English |
| Detected Language | English |
| Type | Thesis |
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