Streptococcus suis (S. suis) is an important opportunistic pathogen, which can cause
septicemia and meningitis in pigs and humans. Previous in vivo observations in S. suisinfected
pigs revealed lesions at the choroid plexus (CP). In vitro experiments with primary
porcine CP epithelial cells (PCPEC) and human CP epithelial papilloma (HIBCPP) cells
demonstrated that S. suis can invade and traverse the CP epithelium, and that the CP
contributes to the inflammatory response via cytokine expression. Here, next generation
sequencing (RNA-seq) was used to compare global transcriptome profiles of PCPEC and
HIBCPP cells challenged with S. suis serotype (ST) 2 infected in vitro, and of pigs infected
in vivo. Identified differentially expressed genes (DEGs) were, amongst others, involved in
inflammatory responses and hypoxia. The RNA-seq data were validated via quantitative
PCR of selected DEGs. Employing Gene Set Enrichment Analysis (GSEA), 18, 28, and 21
enriched hallmark gene sets (GSs) were identified for infected HIBCPP cells, PCPEC, and
in the CP of pigs suffering from S. suis ST2 meningitis, respectively, of which eight GSs
overlapped between the three different sample sets. The majority of these GSs are
involved in cellular signaling and pathways, immune response, and development,
including inflammatory response and hypoxia. In contrast, suppressed GSs observed
during in vitro and in vivo S. suis ST2 infections included those, which were involved in
cellular proliferation and metabolic processes. This study suggests that similar cellular
processes occur in infected human and porcine CP epithelial cells, especially in terms of
inflammatory response.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:84533 |
| Date | 03 April 2023 |
| Creators | Lauer, Alexa N., Scholtysik, Rene, Beineke, Andreas, Baums, Christoph Georg, Klose, Kristin, Valentin-Weigand, Peter, Ishikawa, Hiroshi, Schroten, Horst, Klein-Hitpass, Ludger, Schwerk, Christian |
| Publisher | Frontiers Research Foundation |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 2235-2988, 639620 |
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