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Previous issue date: 2013-03-26 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / The development of new vaccines in the control of various diseases in cattle has been increasing the marketing of animals and animal products. Thus we tested two vaccines, a biotherapy homeopathicvaccine and other recombinantallopathic vaccine,using mycobacteria in their formulations that were subsequently tested in mice and cattle. In order to study the prophylactic effect of homeopathic vaccine and the potency to be used as a vaccine, we used a model of immunizations and infections. To this end, we used mice female of BALB / c lineage which were distributed in 15 groups of three animals each. To assess the possible immune mechanisms involved in homeopathic biotherapy vaccinations we used Mycobacterium massiliense. The biotherapics were prepared from mycobacterial M. massiliense. After infections and immunizations, the animals were euthanized and their livers and spleens were harvested, macerated, homogenized, plated and incubated at 37 ° C for five days. Then we did the counting of colony forming units (CFU) of bacteria recovered from organs and according to the results obtained were selected the potencies11cH and 19cH to be tested as vaccine, because they have shown more homogeneous results. In the animals that were immunized with 19 cHthere were induction of IgG2a class antibodies specific to M. massiliense similar to (0.18 ± 0.07) infections alone (0.19 ± .02). To assess allopathic vaccine protection was used mycobacterium (Mycobacterium smegmatis mc2155 with PLA71/Fusion) in cattle. After allopathic vaccinations, blood was collected and serum was removed for ELISA test. Animals that received the recombinant live vaccine expressing protein of fusion showed greater levels of specific antibodies (p <0.01). This study evaluated the effect ofhomeopathic biotherapy vaccine composed of M. massiliense and allopathicvaccine formulated with M.smegmatis recombinant in different animal models, thus concluding that both vaccines and vaccines homeopathic and allopathic using different kinds of mycobacteria can induce humoral immune response in an animal model. / O desenvolvimento de novas vacinas no controle de várias doenças na bovinocultura vem incrementando a comercialização de animais e produtos de origem animal. Com isso testaram-se duas vacinas, uma vacina bioterápica homeopática e outra vacina alopática recombinante, utilizando micobacterias em suas formulações que posteriormente foram testadas em camundongos e bovinos. Com o objetivo de estudar o efeito profilático da vacina homeopática e a potência a ser utilizada como vacina, foi empregado um modelo de imunizações e infecções. Para tal, utilizou-se camundongos fêmeas da linhagem BALB/c as quais foram distribuídas em 15 grupos com três animais cada. Para avaliar os possíveis mecanismos imunológicos envolvidos nas vacinações bioterápicas homeopáticas utilizou-se Mycobacterium massiliense. Os bioterápicos foram preparados a partir de micobactérias M. massiliense. Após as imunizações e infecções, os animais foram eutanaziados e deles colheram-se os fígados e baços, os quais foram macerados, homogeneizados, plaqueados e incubados a 37ºC durante 5 dias. Em seguida, fez-se a contagem de unidades formadoras de colônias (UFC) das bactérias recuperadas dos órgãos e de acordo com os resultados obtidos foram selecionadas as potências 11cH e 19cH para serem testadas como vacina, por apresentarem resultados mais homogêneos. Nos animais imunizados com 19 cH houve indução da produção de anticorpos da classe IgG2a específicos para M. massiliense semelhantes à (0,18 ± 0,07) infecção sozinha (0,19 ± 0,02). Para avaliar a proteção da vacina alopática, foi utilizada a micobactéria (Mycobacterium smegmatis mc2155 com PLA71/Fusão), em bovinos. Após as vacinações alopáticas foi coletado sangue e retirou-se o soro para o teste de ELISA. Animais que receberam a vacina viva recombinante expressando a proteína de fusão apresentaram níveis maiores de anticorpos específicos (p< 0,01). Com este estudo avaliou-se os efeitos da vacina bioterápica homeopática composta de M. massiliense e de vacina alopática formulada com M. smegmatis recombinante em diferentes modelos animais, concluindo assim que tanto as vacinas homeopáticas e vacinas alopáticas usando diferentes tipos de micobactérias podem induzir resposta imune humoral em modelo animal.
Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.bc.ufg.br:tede/3148 |
Date | 26 March 2013 |
Creators | Cavalcanti, Marcos Antônio Rocha |
Contributors | Junqueira-Kipnis, Ana Paula, Nunes, Romão da Cunha, Rezende, Cintia Silva Minafra e, Junqueira-Kipnis, Ana Paula, Alves Jr, José Roberto Ferreira, Resi, Michelle Guerreiro dos, Fioravanti, Maria Clorinda Soares, Martins, Márcio Eduardo Pereira |
Publisher | Universidade Federal de Goiás, Programa de Pós-graduação em Ciência Animal (EVZ), UFG, Brasil, Escola de Veterinária e Zootecnia - EVZ (RG) |
Source Sets | IBICT Brazilian ETDs |
Language | Portuguese |
Detected Language | English |
Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
Format | application/pdf |
Source | reponame:Biblioteca Digital de Teses e Dissertações da UFG, instname:Universidade Federal de Goiás, instacron:UFG |
Rights | http://creativecommons.org/licenses/by-nc-nd/4.0/, info:eu-repo/semantics/openAccess |
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