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Previous issue date: 2009-06-26 / This experiment aimed at recognizing and quantifying the possible macro and microscopic
tissue alterations that may occur with the application of bovine pericardium preserved in
different concentrations of glutaraldehyde on the abdominal wall. Besides, it also intended to
identify the possible interferences in the implant incorporation, according to the variation in
the concentration of each solution. Fresh bovine pericardium, after cleaning and the removal
of fat, were fractioned and treated with 98% glycerin solution for 30 days (control groups) or
with 0.625%, 1% and 1.5% glutaraldehyde solution for 18 days (experimental groups). The
fragments were implanted on the abdominal wall of 60 mice (n=15 per treatment) for 7, 14
and 30 days. After each of these periods, five animals of each group were sacrificed for
collection, and macro and microscopic analysis of tissue samples. It was observed that the
inflammatory reaction in the groups treated with glycerin and 0.625% glutaraldehyde was
similar in terms of inflammatory cells infiltration and the repairing process. The groups whose
implants were treated with 1% glutaraldehyde chronically presented (30 days) not only more
intense fibrosis and necrosis, when compared to other glutaraldehyde groups, but also tissue
destruction. These were also observed in the animals that received the pericardium treated
with 1.5% glutaraldehyde, analyzed after 14 and 30 days, what leads to the conclusion that
this concentration is unviable for the treatment of biological membranes. / Este experimento teve como objetivo reconhecer e quantificar as poss?veis altera??es
teciduais macro e microsc?picas que podem ocorrer ap?s a aplica??o de peric?rdio bovino
conservado em diferentes concentra??es de glutaralde?do em parede abdominal. Tamb?m
foram identificadas poss?veis interfer?ncias na incorpora??o do enxerto de acordo com a
varia??o da concentra??o da solu??o de tratamento. Peric?rdios bovinos frescos, ap?s limpeza
e retirada de gordura, foram fracionados em fragmentos de 1cm2 e tratados com solu??o de
glicerina 98% por 30 dias (grupos controle) ou com solu??o de gutaralde?do 0,625%, 1% e
1,5% por 18 dias (grupos experimentais). Os fragmentos foram implantados na parede
abdominal de 60 camundongos (n=15 por tratamento) por 7, 14 e 30 dias e ap?s estes
per?odos, cinco animais de cada grupo foram sacrificados para coleta e an?lise macro e
microsc?pica das amostras teciduais. Observou-se que a resposta inflamat?ria dos grupos com
tratamento com glicerina e com glutaralde?do 0,625% foi semelhante tanto na infiltra??o das
c?lulas inflamat?rias quanto no processo de repara??o. Os grupos com implantes tratados com
glutaralde?do 1% apresentaram cronicamente (30 dias) a presen?a de fibrose e necrose mais
intensa na musculatura da parede abdominal, quando comparado com os outros grupos de
glutaralde?do, al?m de destrui??o tecidual. Esta tamb?m foi observada nos animais que
receberam peric?rdio tratado com glutaralde?do 1,5% e foram analisados com 14 e 30 dias, o
que permitiu a conclus?o de que esta concentra??o ? invi?vel para o tratamento de membranas
biol?gicas.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:tede/911 |
| Date | 26 June 2009 |
| Creators | Costa, Cl?udia Borges da |
| Contributors | Andrade, Gisele Braziliano de, Silva, Marta Fernanda Albuquerque da |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Curso de P?s-Gradua??o em Medicina Veterin?ria, UFRRJ, Brasil, Cl?nica e Cirurgia Veterin?ria |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
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