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Previous issue date: 2013-09-13 / In this study we investigated the immunodetection of VEGF, ANG-1 and ANG-2, as well as the number of blood vessels in specimens of reticular (ROLP) and atrophic-erosive oral lichen planus (AEOLP). In addition, were included the control groups oral epithelial hyperplasia (OFH), oral epithelial dysplasia (OED) and oral squamous cell carcinoma (OSCC) in order to find a relation between those angiogenics growth factors and biological behavior. Biopsy specimens diagnosed at the Oral Medicine Division of PUCRS, Brazil, were selected and distributed into the following groups ROLP (n=21); AEOLP (n=11); OFH (n=10); OED (n=10); and OSCC (n=10). They were subjected to immunohistochemical processing with primary antibodies anti-VEGF, anti-ANG-1 and anti-ANG-2. The results do not revelead significant difference in the immunodetection of ANG-1 and ANG-2 between the OLP groups. The ROLP group showed significantly higher immunodetection of VEGF (P=0.01) compared with the AEOLP group. There was no significant difference in the number of blood vessels between the OLP groups (P=0.393). In comparative analysis of OLP with control groups, ROLP and AEOLP cases were considered together, forming the OLP group (n=32). The comparison of immunodetection of those growth factors between the OLP group and each control group showed significantly higher values of ANG-1 in the OLP group compared to the OFH group (P=0.043) and no significant difference in ANG-2. VEGF immunodetection in the OLP group was significantly higher than in the OFH group (P=0.003) and significantly lower compared to the OSCC group (P=0.032). No correlation was observed between growth factors evaluated in the different groups studied. The results of immunodetection of VEGF, ANG-1 and ANG-2 and the lack of significant difference in the number of blood vessels between ROLP and AEOLP groups indicate that angiogenesis in OLP can not be associated to the different clinical forms of this disease. The lack of correlation between the growth factors in the different lesions indicates the involvement of other pro-angiogenic agents in vascular neoformation and reflects the complexity of this process. Furthermore, immunodetection of growth factors evaluated was similar between OLP and OED, suggesting that angiogenesis in OLP may behave similarly to the epithelial dysplastic lesions. / Neste estudo foi investigada a imunodetec??o do VEGF e das angiopoietinas (ANG) 1 e 2, bem como o n?mero de vasos sangu?neos em esp?cimes de l?quen plano oral reticular (LPOR) e atr?fico-erosivo (LPOAE). Al?m disso, foram inclu?dos os grupos-controle hiperplasia fibroepitelial (HFO), displasia epitelial (DEO) e carcinoma espinocelular (CEO) orais, a fim de encontrar uma rela??o entre esses fatores de crescimento angiog?nicos e comportamento biol?gico. Foram obtidos esp?cimes de bi?psias do arquivo do Servi?o de Estomatologia e Preven??o do C?ncer Bucomaxilofacial do Hospital S?o Lucas da PUCRS, os quais foram distribu?dos nos grupos LPOR (n=21); LPOAE (n=11); HFO (n=10); DEO (n=10); CEO (n=10) e, posteriormente, submetidos ao processamento imunoistoqu?mico com os anticorpos prim?rios anti-VEGF, anti-ANG-1 e anti-ANG-2. Os resultados n?o revelaram diferen?a significativa na imunodetec??o da ANG-1 e ANG-2 entre os grupos l?quen plano oral (LPO). No entanto, o grupo LPOR apresentou imunodetec??o significativamente superior do VEGF (p=0,01) comparado ao grupo LPOAE. Com rela??o ao n?mero de vasos sangu?neos, a compara??o entre os grupos LPO n?o mostrou diferen?a significativa entre ambos (p=0,393). Na an?lise comparativa com os grupos-controle, os casos de LPOR e LPOAE foram considerados em conjunto, formando o grupo LPO (n=32). A compara??o da imunodetec??o dos fatores de crescimento entre o grupo LPO com cada grupocontrole revelou valores significativamente superiores da ANG-1 no grupo LPO em rela??o ao grupo HFO (p=0,043) e aus?ncia de diferen?a significativa nos valores da ANG-2. A imunodetec??o do VEGF foi significativamente superior no grupo LPO em rela??o ao grupo HFO (p=0,003) e inferior quando comparada ao grupo CEO (p=0,032). N?o foi observada correla??o entre os fatores de crescimento avaliados nos diferentes grupos estudados. Os resultados observados na imunodetec??o do VEGF, ANG-1 e ANG-2 e a falta de diferen?a significativa no n?mero de vasos entre os grupos LPOR e LPOAE indicam que a angiog?nese no LPO possa n?o estar associada ?s diferentes formas cl?nicas desta doen?a. A falta de correla??o entre estes fatores de crescimento nos diferentes tipos de les?es estudadas indica o envolvimento de outras citocinas pr?-angiog?nicas, al?m daquelas avaliadas neste estudo, na neoforma??o vascular e reflete a complexidade deste processo. Por outro lado, a imunodetec??o dos fatores de crescimento avaliados foi semelhante entre os grupos LPO e DEO, sugerindo que a angiog?nese no LPO possa comportar-se de modo semelhante ao de les?es displ?sicas epiteliais.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/1227 |
| Date | 13 September 2013 |
| Creators | Payeras, M?rcia Rodrigues |
| Contributors | Salum, Fernanda Gon?alves |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Odontologia, PUCRS, BR, Faculdade de Odontologia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -8096554818733665164, 500, 600, 4673435736271820140 |
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