TRPC6, the sixth member of the family of canonical transient receptor potential (TRP)
channels, contributes to a variety of physiological processes and human pathologies. This study
extends the knowledge on the newly developed TRPC6 blocker SH045 with respect to its main target
organs beyond the description of plasma kinetics. According to the plasma concentration-time course
in mice, SH045 is measurable up to 24 h after administration of 20 mg/kg BW (i.v.) and up to 6 h
orally. The short plasma half-life and rather low oral bioavailability are contrasted by its reported
high potency. Dosage limits were not worked out, but absence of safety concerns for 20 mg/kg
BW supports further dose exploration. The disposition of SH045 is described. In particular, a high
extravascular distribution, most prominent in lung, and a considerable renal elimination of SH045
were observed. SH045 is a substrate of CYP3A4 and CYP2A6. Hydroxylated and glucuronidated
metabolites were identified under optimized LC-MS/MS conditions. The results guide a reasonable
selection of dose and application route of SH045 for target-directed preclinical studies in vivo with
one of the rare high potent and subtype-selective TRPC6 inhibitors available
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:89135 |
| Date | 18 January 2024 |
| Creators | Chai, Xiao-Ning, Ludwig, Friedrich-Alexander, Müglitz, Anne, Gong, Yuanyuan, Schäfer, Michael, Regenthal, Ralf, Krügel, Ute |
| Publisher | MDPI |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 3635 |
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