Thesis (MSc)--Stellenbosch University, 2003. / ENGLISH ABSTRACT: In this study, the analysis of ammo acids usmg High Performance Liquid
Chromatography (HPLC) with pre-column derivatisation was optimised. The
derivatisation reagents include o-phthaldialdehyde (OPA), 9-
fluorenylmethylchloroformate (FMOC) and iodoacetic acid (IDA). Detection was
performed using UV and fluorescence in series. The developed method was utilised
for the analysis and quantitation of amino acids in eighteen wines. The application of
chemometric data evaluation was initiated. / AFRIKAANSE OPSOMMING: Hierdie ondersoek behels die optimisering van die aminosuuranalise deur gebruik te
maak van Hoë Druk Vloeistof Chromatografie (HDVC) in kombinasie met pre-kolom
derivatisering. Die derivativatiserings reagense sluit in o-phthaldialdehied (OPA), 9-
fluorenielmetielchloroformaat (FMOC) en jodoasynsuur (IDA). Deteksie is gedoen
deur gebruik gemaak van 'n ultraviolet (UV) en 'n fluorosensie detektor in serie. Die
metode sodoende ontwikkel is gebruik vir die analise en kwantifisering van aminosure
in agtien wyne. Die toepassing van chemometriese data evaluasie is ook geïnisieer.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:sun/oai:scholar.sun.ac.za:10019.1/53249 |
| Date | 12 1900 |
| Creators | Douglas, C. A. (Claire Anne) |
| Contributors | Sandra, P. J. F., Lynen, F., Stellenbosch University. Faculty of Science. Dept. of Chemistry & Polymer Science . |
| Publisher | Stellenbosch : Stellenbosch University |
| Source Sets | South African National ETD Portal |
| Language | en_ZA |
| Detected Language | English |
| Type | Thesis |
| Format | 95 p. : ill. |
| Rights | Stellenbosch University |
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