Polycystic kidney disease is a disorder that leads to fluid filled cysts that replace normal renal tubes.
During the process of cellular development and in the progression of the diseases, fibrocystin
can lead to impaired organ formation and even cause organ defects. Besides cellular polarity,
mechanical properties play major roles in providing the optimal apical-basal or anterior–posterior
symmetry within epithelial cells. A breakdown of the cell symmetry that is usually associated with
mechanical property changes and it is known to be essential in many biological processes such as
cell migration, polarity and pattern formation especially during development and diseases such
as the autosomal recessive cystic kidney disease. Since the breakdown of the cell symmetry can be
evoked by several proteins including fibrocystin, we hypothesized that cell mechanics are altered by
fibrocystin. However, the effect of fibrocystin on cell migration and cellular mechanical properties
is still unclear. In order to explore the function of fibrocystin on cell migration and mechanics,
we analyzed fibrocystin knockdown epithelial cells in comparison to fibrocystin control cells. We
found that invasiveness of fibrocystin knockdown cells into dense 3D matrices was increased and
more efficient compared to control cells. Using optical cell stretching and atomic force microscopy,
fibrocystin knockdown cells were more deformable and exhibited weaker cell–matrix as well as
cell–cell adhesion forces, respectively. In summary, these findings show that fibrocystin knockdown
cells displayed increased 3D matrix invasion through providing increased cellular deformability,
decreased cell–matrix and reduced cell–cell adhesion forces
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:84954 |
| Date | 26 April 2023 |
| Creators | Puder, Stefanie, Fischer, Tony, Mierke, Claudia Tanja |
| Publisher | IOP Publishing |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 1478-3975, 066006 |
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