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Previous issue date: 2017-01-16 / Oral mucosa is continuously subjected to traumatic injuries, being a common site for the occurrence of ulcerated lesions. These lesions are susceptible to infections that may delay healing. In the first article of this dissertation, a review of the literature was performed with the aim of understanding the mechanism of action and use of chlorhexidine, povidone-iodine and erythromycin in the healing process of oral wounds. These substances have particular characteristics, independent of their antimicrobial effect, which could favor the reestablishment of tissue architecture. However, few studies evaluated the effect of these drugs on the healing process of oral ulcers. Thus, the second article presents a study in animal model, which was carried out with the aim of to assessing the effect of 0.12% chlorhexidine, 10% povidone-iodine and 0.5% erythromycin in the repair of traumatic ulcers on ventral tongue. Forty-eight Wistar rats were divided into four groups: control, chlorhexidine, povidone-iodine and erythromycin. A 5-mm-diameter excisional wound was made in the center of ventral tongue. After 24 h, a microbiological sample was taken and daily application of the substances started. In six animals from each group, the applications were carried out for three days and in the others for seven days. Prior to euthanasia a new microbiological collection was performed. The photographic record of the residual lesions was obtained for clinical evaluation and, subsequently, the tongue was resected for histological analysis. In the clinical evaluation of the lesion area, the povidone-iodine and chlorhexidine groups presented significantly lower values compared to the control group after three days of treatment. Although the experimental groups presented higher values in the histomorphometric analysis of the epithelial tissue, there was no significant difference in relation to the control. The erythromycin group exhibited the lowest inflammation scores at seven days of treatment. Povidone-iodine showed the best results in the reduction of microorganisms in both experimental times and aerobic and microaerophilic conditions. Based on the results it is possible to conclude that the antimicrobials used in the present study favor the repair process of the oral wounds and may prevent the occurrence of infections. Among the substances tested, 10% povidone-iodine showed the best clinical, histological and microbiological results. / A mucosa bucal ? constantemente submetida a inj?rias traum?ticas, sendo um s?tio frequente para a ocorr?ncia de les?es ulceradas. Tais les?es s?o suscet?veis a processos infecciosos que podem retardar a cicatriza??o. Neste sentido, no primeiro artigo desta disserta??o, foi realizada uma revis?o da literatura com o objetivo de abordar o mecanismo de a??o e o emprego de clorexidina, iodopovidona e eritromicina no processo de reparo de feridas bucais. Essas subst?ncias apresentam caracter?sticas particulares, independentemente do seu efeito antimicrobiano, que podem favorecer o restabelecimento da arquitetura tecidual. Entretanto, poucas pesquisas avaliam o efeito destes f?rmacos no processo cicatricial de ?lceras traum?ticas bucais. Assim, o segundo artigo apresenta um estudo em modelo animal, que foi realizado com o objetivo de avaliar o efeito da clorexidina 0,12%, iodopovidona 10% e eritromicina 0,5% no reparo de ?lceras traum?ticas em ventre lingual. Quarenta e oito ratos Wistar foram distribu?dos em um grupo-controle e tr?s grupos experimentais: clorexidina, iodopovidona e eritromicina. Uma ?lcera com 5 mm de di?metro foi confeccionada no ventre lingual de cada animal. Ap?s 24 h, foi realizada a primeira coleta microbiol?gica e iniciou-se a aplica??o di?ria das subst?ncias. Em seis animais de cada grupo, as aplica??es foram realizadas durante tr?s dias e, nos demais, durante sete dias. Previamente ? eutan?sia uma nova coleta microbiol?gica foi realizada. O registro fotogr?fico das les?es remanescentes foi obtido para avalia??o cl?nica e, posteriormente, a l?ngua dos animais foi ressecada para an?lise histol?gica. Na avalia??o macrosc?pica da ?rea das les?es os grupos iodopovidona e clorexidina apresentaram valores significativamente inferiores aos do grupo-controle ap?s tr?s dias de tratamento. Embora os grupos experimentais tenham apresentado valores superiores quanto ? ?rea de tecido epitelial neoformado, n?o houve diferen?a significativa em rela??o ao controle. O grupo eritromicina exibiu os menores escores de inflama??o aos sete dias de tratamento. A iodopovidona apresentou os melhores resultados na redu??o de micro-organismos em ambos os tempos experimentais, tanto em condi??es de aerobiose, quanto em microaerofilia. Pode-se concluir que os antimicrobianos utilizados neste estudo, favorecem o processo cicatricial e podem prevenir a ocorr?ncia de infec??es. Dentre as subst?ncias testadas, a iodopovidona 10% apresentou os melhores resultados cl?nicos, histol?gicos e microbiol?gicos.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/7183 |
| Date | 16 January 2017 |
| Creators | Teixeira, Dieni da Silveira |
| Contributors | Salum, Fernanda Gon?alves, Oliveira, Silvia Dias de |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Odontologia, PUCRS, Brasil, Faculdade de Odontologia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -8096554818733665164, 600, 600, 600, 4673435736271820140, -2070498469879244349 |
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