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Previous issue date: 2016-01-12 / With the growing expansion of implant surfaces and the need for
greater speed of osseointegration, methods of analysis of bone tissue and
implant surface as well as the quality of the bone implant contact (BIC), they are
increasingly being explored by researchers. The aim of this study is to show
detail and describe a bone preservation protocol for making implants samples
with and without implants without any harm or damage, or the bone tissue or
the properties of the metal, preserving the BIC. In a second step, the samples
prepared by the protocol preservation were compared with those made by
histological analysis method for staining for HE, considered the gold standard
for living tissue. Eight samples were used for bone tissue with implants in rabbit
tibia and more four fragments of jaws without implants of the same rabbits.
Samples with and without implants were made by the two protocols, after
preparation of the test specimens, analysis by optical microscopy of
transmission and reflection through scanned images were made. Each protocol
had advantages when the results were analyzed individually in the implants
without samples, becoming further analysis. However, for samples with
implants, analysis only by histological method cannot bring good results, while
the bone preservation method showed excellent results for osseointegration
analysis, bone formation and bone implant interface. It follows that the bone
preservation protocol is an excellent method for analyzing samples with
implants, and can be complementary to histology, in analysis bone samples
without implants. The histological method is not sufficient for analysis of bone
formation and bone implant interface. / Com a crescente expans?o de superf?cies de implantes e a necessidade de
maior velocidade de osseointegra??o, m?todos de an?lise do tecido ?sseo,
an?lises da superf?cie de implantes e a qualidade do contato osso-implante
(BIC) est?o cada vez mais sendo explorados pelos pesquisadores. O objetivo
do presente estudo foi descrever detalhadamente um protocolo de preserva??o
?ssea para confec??o de amostras com e sem implantes, sem causar danos ou
avarias nem ao tecido ?sseo nem ?s propriedades do metal, preservando o
BIC. Em uma segunda etapa, as amostras confeccionadas pelo protocolo de
preserva??o ?ssea foram comparadas com as amostras preparadas pelo
m?todo de an?lise histol?gica por colora??o por Hematoxilina-Eosina,
considerado padr?o ouro para tecidos vivos. Foram utilizadas oito amostras de
tecido ?sseo com implantes de t?bias de coelhos e, mais quatro fragmentos de
mand?bulas sem implantes, dos mesmos coelhos. As amostras com e sem
implantes foram confeccionadas pelos dois protocolos e, ap?s a prepara??o
dos corpos de prova, foram feitas as an?lises por microscopia ?ptica de
transmiss?o e reflex?o atrav?s de imagens digitalizadas. Cada protocolo
apresentou vantagens quando os resultados foram analisados individualmente
nas amostras sem implantes. Por?m, para as amostras com implantes, a
an?lise apenas pelo m?todo histol?gico n?o conseguiu apresentar bons
resultados, enquanto que o m?todo de preserva??o ?ssea mostrou excelentes
resultados para an?lise de osseointegra??o, neoforma??o ?ssea e interface
osso-implante. Conclui-se que o protocolo de preserva??o ?ssea desenvolvido
mostrou-se um excelente m?todo para an?lise de amostras com implantes, e
que pode ser complementar ? histologia, em an?lises de tecido ?sseo sem
implantes. O m?todo histol?gico n?o mostrou-se suficiente para an?lise de
neoforma??o ?ssea e interface osso-implante.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/6568 |
| Date | 12 January 2016 |
| Creators | Kunrath, Marcel Ferreira |
| Contributors | H?bler, Roberto |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Engenharia e Tecnologia de Materiais, PUCRS, Brasil, Faculdade de Engenharia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -7432719344215120122, 600, 600, 600, -655770572761439785, 4518971056484826825 |
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