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Previous issue date: 2014-02-27 / The pathogenesis of nonalcoholic fatty liver disease (NAFLD) is not completely elucidated. There is increasing evidence that oxidative stress is involved in this process. The objective of the present study was to evaluate the role of plasma markers of oxidative stress in morbidly obese patients with NAFLD. Forty-three morbidly obese patients were subjected to Roux-en-Y gastric bypass with a liver biopsy obtained during surgery. Plasma oxidative stress was measured by thiobarbituric acid reactive substances (TBARS) and superoxide dismutase (SOD) assays. Histologic analysis showed NASH in 65.1% of patients and simple steatosis in 34.9%. The plasma TBARS levels were significantly greater in the patients with NASH when compared to those with simple steatosis [1.65 (1.43-1.82) nM/ml vs 1.12 (1.04-1.42) nM/ml; P=0.002]. There was a correlation of TBARS with NAS score (rs = 0.470; P = 0.001). Among the patients with NASH and simple steatosis, there was no difference in plasma antioxidant status measured by SOD. Our findings suggest that plasma lipid peroxidation is associated with the severity of NAFLD in the morbidly obese patients / A patog?nese da doen?a hep?tica gordurosa n?o alco?lica (DHGNA) n?o est? completamente esclarecida. Existem evid?ncias cada vez maiores de que o estresse oxidativo participa desse processo. O objetivo do presente estudo ? avaliar o papel de marcadores plasm?ticos de estresse oxidativo em obesos m?rbidos com DHGNA. Foram inclu?dos 43 obesos m?rbidos submetidos ao bypass g?strico em Y-de-Roux, com bi?psia hep?tica obtida durante a cirurgia. O estresse oxidativo plasm?tico foi mensurado por meio das subst?ncias que reagem ao ?cido tiobarbit?rico (TBARS) e ao super?xido dismutase (SOD). A an?lise histol?gica evidenciou NASH em 65,1% dos pacientes e esteatose simples em 34,9%. Os n?veis plasm?ticos de TBARS foram significativamente maiores nos pacientes com NASH, quando comparados aos com esteatose simples [1,65 (1,43-1,82) nM/ml vs 1,12 (1,04-1,42) nM/ml; P=0,002]. Houve correla??o da TBARS com o NAS score (rs= 0,470; P=0,001). Entre os pacientes com NASH e esteatose simples, n?o houve diferen?a no status antioxidante plasm?tico medido pela SOD. Nossos achados sugerem que a lipoperoxida??o plasm?tica est? associada com a gravidade da DHGNA em obesos m?rbidos
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/1772 |
| Date | 27 February 2014 |
| Creators | Maggioni, Lucas Spadari |
| Contributors | Padoin, Alexandre Vontobel |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Medicina e Ci?ncias da Sa?de, PUCRS, BR, Faculdade de Medicina |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 7620745074616285884, 500, 600, -8624664729441623247 |
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