Wong, Yee Man Melody. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2009. / Includes bibliographical references (leaves 148-174). / Abstract also in Chinese. / Abstract --- p.i / Acknowledgements --- p.iv / Abbreviations --- p.v / Review of the literature --- p.1 / Overview of liver fibrosis --- p.2 / Pathophysiology of liver fibrosis --- p.3 / Histological classification of liver fibrosis --- p.4 / Gold standard for fibrosis assessment - Liver biopsy --- p.6 / Biomarker in blood - non-invasive method for assessing diseases --- p.7 / Significance of non-invasive markers of liver fibrosis --- p.9 / Biomarkers of liver fibrosis --- p.10 / Direct markers --- p.10 / Indirect markers --- p.11 / Proteomics / Why proteomics? --- p.16 / Clinical values of proteomics in biomarker discovery --- p.17 / Challenges in proteomics --- p.18 / Current proteomics technologies in biomarker discovery --- p.21 / Gel based --- p.21 / Gel free approach - MS based --- p.23 / Quantitative proteomics --- p.29 / Application of proteomics to discovery of biomarkers for diagnosis of liver fibrosis --- p.33 / Rationale and Objectives of the Project --- p.35 / Chapter Section 1 --- Method development of magnetic beads-based proteomic profiling for quantitative proteomic profiling and micro- purification in parallel --- p.36 / Chapter 1.1 --- Introduction --- p.36 / Chapter 1.2 --- Materials and methods --- p.38 / Chapter 1.3 --- Results / Chapter 1.3.1 --- Serum proteome profiles obtained with different types of chromatographic magnetic beads --- p.46 / Chapter 1.3.2 --- Performance of PCS 4000 ProteinChip reader --- p.49 / Chapter 1.3.3 --- Reproducibility of magnetic beads-based serum proteomic profiling --- p.54 / Chapter 1.3.4 --- Gel electrophoresis of the eluted proteins --- p.58 / Chapter 1.3.5 --- Identification of the protein peaks --- p.58 / Chapter 1.4 --- Discussion --- p.60 / Chapter 1.5 --- Conclusion --- p.64 / Chapter Section 2 --- Development of a proteome-based fingerprinting model for detecting liver fibrosis in patients with chronic hepatitis B infection --- p.65 / Chapter 2.1 --- Introduction --- p.65 / Chapter 2.2 --- Materials and methods --- p.68 / Chapter 2.3 --- Results / Chapter 2.3.1 --- Patients characteristics --- p.75 / Chapter 2.3.2 --- Correlation between biochemical/serological markers and the degrees of liver fibrosis --- p.81 / Chapter 2.3.3 --- Serum proteomic profiling by linear MALDI-TOF MS --- p.81 / Chapter 2.3.4 --- Correlation of proteomic features with Ishak score --- p.81 / Chapter 2.3.5 --- Correlation of significant proteomic features with serological markers --- p.89 / Chapter 2.3.6 --- Construction of diagnostic model in detecting liver fibrosis and cirrhosis --- p.91 / Chapter 2.3.7 --- Cross-validation of the diagnostic model using pre- treatment samples in detecting liver fibrosis and cirrhosis --- p.91 / Chapter 2.3.8 --- Independent validation of the diagnostic model using post-treatment samples in detecting liver fibrosis and cirrhosis --- p.95 / Chapter 2.3.9 --- Comparison against other non-invasive models in detecting liver fibrosis and cirrhosis --- p.98 / Chapter 2.4 --- Discussion --- p.103 / Chapter 2.5 --- Conclusion --- p.112 / Chapter Section 3 --- Identification of proteomic features to form diagnostic fingerprint for the detection of liver fibrosis in patients with chronic hepatitis B infection --- p.113 / Chapter 3.1 --- Introduction --- p.113 / Chapter 3.2 --- Materials and methods --- p.115 / Chapter 3.3 --- Results --- p.121 / Chapter 3.3.1 --- Protein identification of the protein marker in the diagnostic model --- p.121 / Chapter 3.3.2 --- Immunodepletion of apolipoprotein C-III --- p.125 / Chapter 3.3.3 --- Serum levels of apolipoprotins and their association with liver fibrosis --- p.127 / Chapter 3.4 --- Discussion --- p.130 / Chapter 3.5 --- Conclusion --- p.139 / General discussion --- p.141 / Reference --- p.148 / Original Data --- p.175
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_326937 |
| Date | January 2009 |
| Contributors | Wong, Yee Man Melody., Chinese University of Hong Kong Graduate School. Division of Medical Sciences. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, viii, 175 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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