The experiment was done at Faculty of Horticulture in Lednice, at the Mendeleum-Institute of genetics. The plant materil was obtained from the technical isolation in Lednice. Four selected grape varieties ('MT' 25/7, 20/52'PM','CR2' 1/48,'K 5BB') as primary culture and multiplied in selected media. All varieties were grown at the same temperature and light conditions. MS medium combined with BA (0.7 mg.l-1), IAA (0.1 mg.l-1) was chosen for the primary culture. The pH was adjusted to 5.8. After the establishment of primary cultures, only the variety 'CR 2' 1/48 survived. In the other varieties ('MT' 07/25, 20/52 and 'PM''K 5BB') infection by fungal and bacterial infections, hyperhydratation, no proliferation of shoots and necrosed individual segments were observed. Variety'CR2' was (after the establishment of primary cultures) multiplied onfive media; DKW combination of BA (0.6 mg.l-1), IBA (0.01 mg.l-1), MS in combination with BA (3.0 mg.l-1), NAA (0, 2 mg.l-1), C2D combination of BA (1.5 mg.l-1), MS enriched by BA (1.5 mg.l-1), IBA (1 0 mg.l-1), and LQ combination of BA (0.4 mg.l-1) and NAA (0.01 mg.l-1). Individual media were evaluated separately with the calculation multiplier of 4 passage. C0 medium was evaluated as the most suitable medium for in vitro grapevine multiplication . The lowest number of new formed shoots and also the worst results were observed on C1 medium. The results of the thesis evaluaete the health condition of plants and the multiplication factor on different media. New formed shoots during the ever single passaging was evaluated as well.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:363110 |
| Date | January 2016 |
| Creators | Mančíková, Simona |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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