(1) Background: Canine distemper virus (CDV)-induced demyelinating leukoencephalitis
(CDV-DL) in dogs and Theiler’s murine encephalomyelitis (TME) virus (TMEV)-induced
demyelinating leukomyelitis (TMEV-DL) are virus-induced demyelinating conditions mimicking
Multiple Sclerosis (MS). Reactive oxygen species (ROS) can induce the degradation of lipids and nucleic
acids to characteristic metabolites such as oxidized lipids, malondialdehyde, and 8-hydroxyguanosine.
The hypothesis of this study is that ROS are key eector molecules in the pathogenesis of myelin
membrane breakdown in CDV-DL and TMEV-DL. (2) Methods: ROS metabolites and antioxidative
enzymes were assessed using immunofluorescence in cerebellar lesions of naturally CDV-infected
dogs and spinal cord tissue of TMEV-infected mice. The transcription of selected genes involved
in ROS generation and detoxification was analyzed using gene-expression microarrays in CDV-DL
and TMEV-DL. (3) Results: Immunofluorescence revealed increased amounts of oxidized lipids,
malondialdehyde, and 8-hydroxyguanosine in CDV-DL while TMEV-infected mice did not reveal
marked changes. In contrast, microarray-analysis showed an upregulated gene expression associated
with ROS generation in both diseases. (4) Conclusion: In summary, the present study demonstrates
a similar upregulation of gene-expression of ROS generation in CDV-DL and TMEV-DL. However,
immunofluorescence revealed increased accumulation of ROS metabolites exclusively in CDV-DL.
These results suggest dierences in the pathogenesis of demyelination in these two animal models.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:89017 |
| Date | 15 January 2024 |
| Creators | Attig, Friederike, Spitzbarth, Ingo, Kalkuhl, Arno, Deschl, Ulrich, Puff, Christina, Baumgärtner, Wolfgang, Ulrich, Reiner |
| Publisher | MDPI |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 3217 |
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