This research studied the dietary effect of supplemental plant oils on growth, adiposity and lipid metabolism-related enzyme activity of juvenile cobia. The isonitrogenous and isoenergetic basal diet contained 15% crude lipid, 6% fish oil and 9% supplemental oils. The supplemental oils were varied among 5 dietary treatments, including fish oil (HUFA, n-3)(FO), perilla oil (18:3 n-3)(PE), safflower oil (18:2 n-6)(SA), olive oil (18:1 n-9)(OL), and palm oil (16:0)(PA). Results of the 10-wk feeding trial show that fish fed diet containing palm oil had the highest final weight, and was significant higher than fish fed SA diet. SA group had highest crude lipid concentration. OL group had the least crude protein concentration. PA group had the highest ash concentration. FO group had the highest crude protein and moisture content, the least crude lipid and ash content. Adipocyte density in various, tissues did not vary with time, except the ventral fat depot. Tissue adipocyte density of FO group was the least. Adipocyte density of PE group was higher than FO group, and its mean adipcoyte diameter in dorsal muscle was great than the other groups. Fatty acid synthase (FAS), as measured by specific activity, decreased with times in the PE group. Adipocyte density of SA group the highest of all groups, and its mean adipocyte diameter in dorsal muscle was also the greatest. Adipocyte density in the ventral fat depot of OL group was the highest, and its mean diameter in dorsal muscle was the smallest. Adipocyte density of PA group was only slightly lower than SA group. The tissue acid composition of the cobia was influenced by the supplemental plant oils. Tissue HUFA concentration and n-3/n-6 ratio was decreased, MUFA, PUFA and SAF composition was increased when the plant oils were supplemented. The results show that the supplementation of the plant oils could affect the density , size and tissue distribution of adipocytes, fatty acid synthesis pathway in the liver and tissue fatty acid composition. Feeding the cobia diet containing supplemental safflower or palm oil significantly increased density and cell size of adipocytes in the tissue of the cobia.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0815108-161518 |
| Date | 15 August 2008 |
| Creators | Lin, Pei-Chen |
| Contributors | Shi-Yen Shiau, Houng-Yung Chen, Li-Lian Liu |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0815108-161518 |
| Rights | unrestricted, Copyright information available at source archive |
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