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Fungal Antigens and Fungal Disease: An Alkali-Soluble, Water Soluble Antigen from Coccidioides immitis and Coccidioidomycosis

Diagnostic medical mycology has been slow to advance due to a lack of species specific antigens in organisms which cause serious diseases in man. Toward this end, an HPLC analysis was done of the following fungal antigens: histoplasmins HKC-43 and H-42, blastomycin KCB-26, an alkali-soluble, water soluble antigen from Blastomyces dermatitidis (b-ASWS), a coccidioidin prepared from a toluene lysate of the mycelial-arthroconidia phase of Coccidioides immitis, and an alkali-soluble, water-soluble antigen from Coccidioides immitis (c-ASWS). The HPLC survey included size-exclusion chromatography (SEC), ion exchange chromatography (HPIEC), and reversephase chromatography (RP). Resolution was poor with both SEC and HPIEC but was excellent with RP chromatography. The use of RP will allow sufficient separation for further antigenic and structural analysis.

Identiferoai:union.ndltd.org:unt.edu/info:ark/67531/metadc331669
Date12 1900
CreatorsFleming, William H. (William Harold)
ContributorsVela, G. Roland, 1927-, Gracy, Robert W., McDonald, W. C., Johansson, Karl R.
PublisherNorth Texas State University
Source SetsUniversity of North Texas
LanguageEnglish
Detected LanguageEnglish
TypeThesis or Dissertation
Formatviii, 204 leaves : ill., Text
RightsPublic, Fleming, William H. (William Harold), Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved.

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