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Previous issue date: 1997-09-30 / CNPq / This experimental work with Aedes aegypti L. (1762) was conducted in order to compare the effect of differents blood sources on feeding and egg production of mosquitoes? females. The blood-feeding device consisted of a plastic cage ? used for holding females during blood feeding ? with two lateral windows recovered with thin cloth to allow air exchange during feeding. In the top of the cage, there was an opening where the food?s container, recovered with silicone membrane, was attached. A larger vial filled with water at the initial temperature of 50 ?C was used over the apparatus to keep the food source warm (37,5 ? 0,5%). In each trial, three different treatments were tested: whole blood collected from mouse, bovine and human. To prevent coagulation, a 3,9% solution of sodium citrate was added to the blood. The control group was allowed to feed directly on human volunteers. The experiment was conducted in a climatic chamber, with temperature and humidity controlled at 28?0,5 ?C and 80?5%, respective values and the photoperiod of 12 hours of light and 12 hours of darkness. In each tested group, 30 females and 10 males mosquitoes were used. The females were allowed to feed during a period of 20-30 minutes and after that only the engorged females were transferred to another cages containing the oviposition sites. To evaluate the effect of different sources of blood on A. aegypti, the percentage of engorged females, pre-oviposition time and the number of eggs per engorged female were measured. Highest percentage of engorged females were among mosquitoes fed on human citrated blood (89%) and control (95%). The media oviposition on both treatments were 13,4 eggs for females fed on human citrated blood and 15,3 eggs for females of control group. The lots fed on mice and bovine citrated blood presented the following results respectively: 22,5% of engorged females and 9,2 eggs per female and 25,8% of engorged females and 10,3 eggs per female. / Foi comparado o efeito do sangue de camundongos, bovino e humano sobre o ingurgitamento e oviposi??o do mosquito Aedes aegypti L., 1762 (D?ptera:Culicidae). O aparato para alimenta??o artificial foi constitu?do por gaiolas de pl?stico, com 10 x 6 cm, com orif?cios laterais cobertos com tela de tule, e uma abertura superior, atrav?s da qual foi oferecido o sangue contido em uma c?lula montada em l?mina de microscopia com cola de silicone e membrana de silicone. O sangue foi mantido aquecido a 37,5 ? 0,5 ?C, durante o tempo de alimenta??o que variou de 20 a 30 minutos. O experimento foi conduzido dentro de uma c?mara climatizada, com temperatura de 28 ? 0,5 ?C, umidade relativa de 80 ? 5% e fotoperiodismo de 12 horas di?rias. Um total de 16 grupos de 30 f?meas de A. aegypti, com idades entre 4 a 7 dias, foram pr?-alimentados com solu??o a?ucarada de sacarose. Em cada grupo foram adicionados 10 machos para realiza??o de c?pula. O sangue de cada hospedeiro foi coletado assepticamente e adicionado de citrato de s?dio a 3,9%. O grupo controle foi alimentado diretamente em hospedeiro humano volunt?rio. Ap?s a alimenta??o, as f?meas ingurgitadas foram contadas e colocadas no interior de gaiolas com 50 x 50 x 50 cm, contendo frasco com ?gua limpa para oviposi??o. Foram registrados o n?mero de f?meas alimentadas em cada tratamento, o per?odo de pr?-postura e o n?mero m?dio de ovos por f?mea alimentada. Mosquitos alimentados com sangue de camundongos e de bovino tiveram performances de 22,5 e 25,8% de ingurgitamento e a postura foi em m?dia de 9,2 e 10,3 ovos por f?mea, respectivamente. F?meas alimentadas com sangue humano atrav?s da membrana ou diretamente no hospedeiro humano tiveram comportamentos diferentes com ?ndices de ingurgitamento de 89 e 95% e oviposi??o de, em m?dia, 13,4 e 15,3 ovos por f?mea, respectivamente. N?o houve varia??o no per?odo de pr?-postura (tr?s dias) entre os tratamentos realizados.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:jspui/1428 |
| Date | 30 September 1997 |
| Creators | PINA, Isabelle Garcia |
| Contributors | Fonseca, Adivaldo Henrique da, Fonseca, Adivaldo Henrique da, Bittencourt, V?nia Rita Elias Pinheiro, Faccini, Jo?o Luiz Hor?cio |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Programa de P?s-Gradua??o em Ci?ncias Veterin?rias, UFRRJ, Brasil, Instituto de Veterin?ria |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | BAR-ZEEV, M. & SMITH, C.N. 1959. Action of repellents on mosquitoes feeding through treated membranes or on treated blood. Jour. Econ. Ent., 52:263-267. BEHIN, R. 1967. Artificial feeding apparatus for mosquitoes. Mosquito News. 27:87-90. BISHOP, A. & GILCHRIST, B.M. 1944. Method for collecting sporozoites of Plasmodium gallinaceum by feeding infected Aedes aegypti through animal membranes. Nature. 3893:713-714. BISHOP, A. & GILCHRIST, B.M. 1946. Experiments upon the feeding of Aedes aegypti through animal membranes with a view to applying this method to the chemotherapy of malaria. Parasitology, 35:85-100. BOORMAN, J.P.T. 1958. Transmission of Uganda S virus by Aedes (Stegomyia) aegypti LINN. Trans. R. Soc. Trop. Med. Hyg., 52:383-388. BOORMAN, J.P.T. & PORTERFIELD, J.S. 1956. A simple technique for infection of mosquitoes with viruses. Trans. R. Soc. Trop. Med. Hyg., 50:238-242. BUTLER, J.F.; HESS, W.R.; ENDRIS, R.G. & HOLCHER, K.H. 1984. In vitro feeding of Ornithodoros ticks for rearing and assessment of diseases transmission pp. 1075-1081. In D.A. Griffiths and C.E. Bowman (ed.), Acarology VI, Vol 2. Ellis Horwood, West Sussex, England. CONSOLI, R.A.G.B. & OLIVEIRA, R.L. 1994. Principais mosquitos de import?ncia sanit?ria no Brasil. Rio de Janeiro: Fiocruz. 228p. DAVIS, E.L.; BUTLER, J.F.; ROBERTS, R.H.; REINERT, J.F. & KLEINE, D.L. 1983. Laboratory blood feeding of Culicoides mississippiensis (Diptera: Ceratopogonidae) through a reinforced membrane. J. Med. Entomol. 20:177-182. DUTRA, A.E. 1995. Alimenta??o artificial de Argas (persicargas) miniatus (Koch, 1844) (Acari:Argasidae) atrav?s de membrana de silicone. Tese de Mestrado. Universidade Federal Rural do Rio de Janeiro. 42 p. FRIEND, W.G. & SMITH, J.J.B. 1977. Factors affecting feeding by bloodsucking insects. Ann. Rev. Entomol. 22:309-331. GALUN, R. 1967. Feeding stimuli and artificial feeding. Bull. Wld. Hlth. Org. 36:590-593. GALUN, R. & RICE, M.J. 1971. Role of blood platelets in haematophagy. Nature New Biology. 233:110-111. GALUN, R; AVI-DOR, Y. & BAAR-ZEEV, M. 1963. Feeding response in Aedes aegypti: stimulation by Adenosine Triphosphate. Science. 142:1674-75. GALUN, R.; KOSOWER, E.M. & KOSOWER, N.S. 1969. Effect of methyl phenyldiazenacarboxylate (Azooter) on the feeding behavior of bloodsucking invertebrates. Nature. 224-182. GERBERG, E.J. 1970. Manual for mosquito rearing and experimental techniques. Amer. Mosquito Control Assoc. Bul. 5:1-109. GREENBERG, J. 1949. A method for artificially feeding mosquitoes. Mosquito News. 9:48-50. KEMP, D.H.; KOUDSTAAL, D.; ROBERTS, J.A. & KERR, J.D. 1975. Feeding of Boophilus microplus larvae on a partially defined medium through thin slice of cattle skin. Parasitology. 70:243-254. LEHANE, M.J. 1991. Biology of blood-sucking insects. London: Harper Collins Academic. 228p. MACDONALD, E. & SCOTT, J.A. 1952. Methods of feeding tropical rat mites on blood and other fluids through a membrane. Exp. Parasitol. 1:283-290. 14 McCLELLAND, G.A.H. 1974. A worldwide survey of variation in scale pattern of the abdominal tergum of Aedes aegypti (L.) (Diptera: Culicidae). Trans. R. Entomol. Soc. London, 126:239-259. MOURA, S.T.; FONSECA, A.H.; FERNANDES, C.G. & BUTLER, J. 1997. Artificial feeding Amblyomma cajennense (Fabricius, 1787) (Acari: Ixodidae) through silicone membrane. Mem. Inst. Oswaldo Cruz, 92:545-548. OGSTON, C.W. & YANOVSKY, A.D. 1982. An improved artificial feeder for bloodsucking insects. J. Med. Entomol. 19:42-44. PIERCE, A.E. & PIERCE, M.H. 1956. A note on the cultivation of Boophilus microplus (Canestrini, 1887) (Ixodidae: Acarina) on the embrionated hen egg. Aust. Vet. J. 32:144-146. PIPKIN, A.C. & CONNOR, J.C. 1968. A temperature controlled feeding apparatus for hematophagous arthropods. J. Med. Ent. 5:507-509. RODHAIN, J.; PONS, C.; VANDENBRANDEN, J. & BEQUAERT, J. 1912. Contribution au mecanisme de la transmission des trypanosomes par les glossines. Arch. Schiffs-u. Tropen-Hyg. 16:732-739. ROCHA, D.S.; FONSECA, A.H.; COSTA, F.A.; JURBERG, J. & GALV?O, C. 1997. Desenvolvimento de Rhodnius pictipes Stal, 1872 (Hemiptera, Reduvidae, Triatominae) alimentado atrav?s de membrana de silicone e em camundongos. Mem. Inst. Oswaldo Cruz, 92:553-558. ROSS, R.W. 1956. A laboratory technique for studying the insect transmission of animal viruses, employing a bat-wing membrane, demonstrated with two african viruses. J. Hyg. 54:192-200. RUTLEDGE, L.C.; WARD, R.A. & GOULD, D.J. 1964. Studies on the feeding response of mosquitoes to nutritive solutions in a new membrane feeder. Mosquito News. 24:407-419. RUTLEDGE, L.C.; MOUSSA, M.A. & BELLETTI, C.J. 1976 An in vitro blood-feeding system for quantitative testing of mosquito repellents. Mosquito News. 36:283-293. ST. JOHN, J.H.S.; SIMMONS, J.S. & REYNOLDS, F.H.K. 1930. Transmission of dengue virus from infected to normal Aedes aegypti. Amer. J. Trop. Med. 10:23-24. STONE, B.F.; COMMINS, M.A. & KEMP, D.H. 1983. Artificial feeding of the Australian paralysis tick Ixodes holocyclus and collection of paralyzing toxin. Int. J. Parasitol. 13:447-454. WETZEL, H. 1979. Artificial membrane for in vitro feeding of piercing-sucking arthropods. Entomol. Exp. Appl. 25:117-119. WOKE, P.A. 1937. Effects of various blood fractions on egg production of Aedes aegypti Linn. A. J. Hyg. 25:372-380. |
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