Thesis (MSc)--Stellenbosch University, 2005. / ENGLISH ABSTRACT: This study:
I. Investigates the influence of S. frutescens on the binding properties of cytochrome
P450-dependent enzymes in ovine adrenocortical mitochondria and microsomes,
demonstrating that S. frutescens extracts elicit difference spectra and inhibit the
Type 1 difference spectra induced by natural steroids.
II. Indicates inhibition by S. frutescens extracts of the catalytic activity of
cytochrome P450-dependent-17a-hydroylase and cytochrome P450-dependentsteroid-
21-hydroxylase enzymes in ovine adrenocortical microsomes.
III. Describes an assay determining the inhibitory effects of S. frutescens, in COS L
cells, on individual cytochrome P450-dependent enzymes - ovine, baboon and
human cytochrome P450-dependent- L7a-hydroylase, and bovine cytochrome
P450-dependent-21-hydroxylase enzymes.
IV. Demonstrates that the inhibition of elevated plasma glucocorticoid levels in rats
exposed to chronic immobilization stress could possibly be attributed to the
influence of hydrophilic and hydrophobic compounds in S. frutescens on
cytochromes P450-depndent enzymes. / AFRIKAANSE OPSOMMING: Hierdie studie:
I. Ondersoek die invloed van S. frutescens op die bindingseienskappe van
sitochroom P450-afhanklike ensieme in skaapbynier mitokondriale en -
mikrosomale preparate en toon aan dat komponente in S. frutescens ekstrakte
verskil spektra induseer en tipe I verskil spektra van natuurlike steroïede inhibeer.
II. Dui die inhiberende effek van S. frutescens ekstrakte op die katalitiese aktiwiteit
van sitochroom P450-afhanklike-17a-hidroksilase en sitochroom P450-
afhanklike-steroïed- 21-hidroksilase ensieme in skaapbyniermikrosome aan.
III. Beskryf 'n tegniek om die inhiberende effek van S. frutescens op individuele
sitochroom P450-afhankilike ensieme - bobbejaan, skaap en mens sitochroom
P450-afhanklike-17a-hidroksilase, en bees sitochroom P450-afhanklike-steroïed-
21-hidroksilase - in COS 1 selle te bepaal.
IV. Demonstreer dat inhibisie van verhoogde glukokortikoïed plasma konsentrasies
waargeneem in rotte blootgestel aan kroniese immobiliserende stress, moontlik
toegeskryf kan word aan die effek van S. frutescens op die sitochroom P450-
afhanklike ensieme.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:sun/oai:scholar.sun.ac.za:10019.1/50270 |
| Date | 12 1900 |
| Creators | Prevoo, Desiree |
| Contributors | Swart, A. C., Swart, P., Stellenbosch University. Faculty of Science. Dept. of Biochemistry . |
| Publisher | Stellenbosch : Stellenbosch University |
| Source Sets | South African National ETD Portal |
| Language | en_ZA |
| Detected Language | English |
| Type | Thesis |
| Format | 114 p. : ill. |
| Rights | Stellenbosch University |
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