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Studies on ovarian maturation of the shrimp, metapenaeus ensis.

Lo Ting Sze. / Thesis submitted in: December 2002. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (leaves 145-170). / Abstracts in English and Chinese. / Declaration --- p.i / Abstract --- p.ii / Acknowledgements --- p.vii / List of Contents / Contents in Brief --- p.viii / Contents in Detail --- p.ix / List of Figures and Tables / List of Figures --- p.xiii / List of Tables --- p.xvi / Chapter Chapter 1 --- Introduction and literature review General introduction --- p.1 / Chapter 1.1 --- Structural changes in ovary of penaeid shrimp during maturation --- p.3 / Chapter 1.2 --- Biochemical and physiological changes in ovary of penaeid shrimp during maturation --- p.7 / Chapter 1.2.1 --- Variation of protein content --- p.7 / Chapter 1.2.1.1 --- Changes of proteins in ovary of penaeid shrimp during maturation --- p.7 / Chapter 1.2.1.2 --- Biochemical characterization of vitellin --- p.8 / Chapter 1.2.1.3 --- Site of vitellogenin synthesis --- p.10 / Chapter 1.2.2 --- Variation of lipid composition --- p.12 / Chapter 1.2.2.1 --- Role of lipids in ovary of penaeid shrimp --- p.12 / Chapter 1.2.2.2 --- Changes of lipids in ovary of penaeid shrimp during maturation --- p.13 / Chapter 1.2.3 --- Variation of other nutrient contents --- p.16 / Chapter 1.3 --- Endocrine control of ovarian maturation in penaeid shrimp --- p.16 / Chapter 1.3.1 --- Gonad-inhibiting hormone (GIH) --- p.17 / Chapter 1.3.2 --- Crustacean hyperglycemic hormone (CHH) --- p.19 / Chapter 1.3.3 --- X-organ sinus gland complex (XOSG) and CHH neuropeptide family --- p.20 / Chapter 1.3.4 --- Gonad-stimulating hormone (GSH) --- p.22 / Chapter 1.3.5 --- Methyl farnesoate (MF) --- p.23 / Chapter 1.3.6 --- Neurotransmitters --- p.24 / Chapter 1.3.7 --- Other factors --- p.26 / Chapter 1.3.8 --- Androgenic hormone (AH) --- p.27 / Chapter 1.4 --- Ecology and reproductive biology of Metapenaeus ensis --- p.29 / Chapter 1.4.1 --- Identification --- p.29 / Chapter 1.4.2 --- Ecology --- p.29 / Chapter 1.4.3 --- Reproductive biology --- p.30 / Chapter 1.5 --- Objective of research --- p.32 / Chapter Chapter 2 --- "Variation of circulating gonad-inhibiting hormone (GIH) level during ovarian maturation in the shrimp, Metapenaeus ensis" / Chapter 2.1 --- Introduction --- p.34 / Chapter 2.2 --- Materials and methods --- p.35 / Chapter 2.2.1 --- Experimental animals and serum extraction --- p.35 / Chapter 2.2.2 --- Protein content assay --- p.36 / Chapter 2.2.3 --- SDS-polyacrymide gel electrophoresis (SDS-PAGE) --- p.39 / Chapter 2.2.4 --- Western blot --- p.40 / Chapter 2.2.5 --- Purification of IgG from antiserum --- p.42 / Chapter 2.2.6 --- ELISA assay (indirect ELISA) --- p.42 / Chapter 2.3 --- Results --- p.44 / Chapter 2.3.1 --- Experimental animals --- p.44 / Chapter 2.3.2 --- Purification of anti-GIH IgG --- p.46 / Chapter 2.3.3 --- Optimization of indirect ELISA --- p.46 / Chapter 2.3.4 --- Determination of circulating GIH in haemolymph --- p.51 / Chapter 2.4 --- Discussion --- p.55 / Chapter Chapter 3 --- "Identification of genes differentially expressed during ovarian maturation in the shrimp, Metapenaeus ensis" / Chapter 3.1 --- Introduction --- p.60 / Chapter 3.2 --- Materials and methods --- p.61 / Chapter 3.2.1 --- Outline of methodology --- p.61 / Chapter 3.2.2 --- Experimental animals --- p.62 / Chapter 3.2.3 --- Total RNA extraction --- p.62 / Chapter 3.2.4 --- RNA arbitrarily primed PCR (RAP-PCR) --- p.64 / Chapter 3.2.5 --- DNA electrophoresis by agarose gel --- p.68 / Chapter 3.2.6 --- DNA electrophoresis by polyacrylamide gel --- p.68 / Chapter 3.2.7 --- Preparation of DIG-labeled probe from PCR product --- p.69 / Chapter 3.2.8 --- Checking for DIG-labeling yield --- p.69 / Chapter 3.2.9 --- Excision of cDNA library from ovary of Metapenaeus ensis --- p.70 / Chapter 3.2.10 --- PCR screening of insertion sequence --- p.73 / Chapter 3.2.11 --- Dot-blot --- p.75 / Chapter 3.2.12 --- Dot-blot hybridization --- p.76 / Chapter 3.2.13 --- Growth of cell for plasmid preparation --- p.79 / Chapter 3.2.14 --- Permanent culture preparation --- p.80 / Chapter 3.2.15 --- DNA Sequencing --- p.80 / Chapter 3.2.16 --- RNA formaldehyde denaturing gel electrophoresis --- p.82 / Chapter 3.2.17 --- Northern blot --- p.84 / Chapter 3.2.18 --- Northern hybridization --- p.84 / Chapter 3.2.19 --- 5´ة RACE (rapid amplification of cDNA ends) --- p.88 / Chapter 3.3 --- Results --- p.92 / Chapter 3.3.1 --- Experimental animals --- p.92 / Chapter 3.3.2 --- Total RNA extraction --- p.92 / Chapter 3.3.3 --- RNA arbitrarily primed PCR (RAP-PCR) --- p.92 / Chapter 3.3.4 --- Titer of cDNA library from Metapenaeus ensis ovary --- p.95 / Chapter 3.3.5 --- PCR screening of insertion sequence --- p.95 / Chapter 3.3.6 --- Dot-blot hybridization --- p.99 / Chapter 3.3.7 --- DNA sequencing of differentially expressed genes --- p.96 / Chapter 3.3.8 --- Northern analysis --- p.99 / Chapter 3.3.8.1 --- Housekeeping gene - elongation factor 1α --- p.106 / Chapter 3.3.8.2 --- Translationally controlled tumor protein (TCTP) --- p.106 / Chapter 3.3.8.3 --- Cytoskeletal actin --- p.109 / Chapter 3.3.8.4 --- Keratin --- p.109 / Chapter 3.3.8.5 --- Heat shock cognate 70 kD protein (hsc70) --- p.109 / Chapter 3.3.8.6 --- Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) --- p.117 / Chapter 3.3.8.7 --- Arginine kinase --- p.117 / Chapter 3.3.8.8 --- High mobility group 1-like protein (HMGl-like protein) --- p.122 / Chapter 3.3.8.9 --- Nucleoside diphosphate kinase --- p.122 / Chapter 3.4 --- Discussion --- p.122 / Chapter 3.4.1 --- RAP-PCR and dot-blot hybridization --- p.122 / Chapter 3.4.2 --- Functions of differentially expressed genes in ovarian maturation --- p.128 / Chapter 3.4.2.1 --- Translationally controlled tumor protein (TCTP) --- p.128 / Chapter 3.4.2.2 --- Cytoskeletal actin --- p.130 / Chapter 3.4.2.3 --- Keratin --- p.130 / Chapter 3.4.2.4 --- Heat shock cognate 70 kD protein (hsc70) --- p.131 / Chapter 3.4.2.5 --- Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) --- p.134 / Chapter 3.4.2.6 --- Arginine kinase --- p.135 / Chapter 3.4.2.7 --- High mobility group 1-like protein (HMGl-like protein) --- p.136 / Chapter 3.4.2.8 --- Nucleoside diphosphate kinase --- p.137 / Chapter 3.4.3 --- Speculation on the molecular changes in ooctyes during ovarian maturation --- p.139 / Chapter Chapter 4 --- General conclusion --- p.142 / References --- p.145

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_324395
Date January 2003
ContributorsLo, Ting Sze., Chinese University of Hong Kong Graduate School. Division of Biology.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xvi, 170 leaves : ill. (some col.) ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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