by Ka-ming Leung. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1991. / Includes bibliographical references. / ABSTRACT --- p.i / ACKNOWLEDGEMENTS --- p.iv / LIST OF CONTENTS --- p.v / LIST OF FIGURES --- p.vii / LIST OF ABBREVIATIONS --- p.xiii / Chapter CHAPTER I --- Introduction --- p.1 / Chapter CHAPTER II --- Literature Review / Chapter 1. --- Digestive System in Decapod Crustaceans --- p.3 / Chapter 2. --- Hepatopancreas in Decapod Crustaceans --- p.7 / Chapter 3. --- Digestive Enzymes from the Hepatopancreas --- p.11 / Chapter 4. --- Changes in the Hepatopancreas during the Digestive Cycle --- p.12 / Chapter 5. --- Effects of Starvation on Hepatopancreas --- p.14 / Chapter 6. --- Biology of Shrimp Metapenaeus ensis --- p.16 / Chapter CHAPTER III --- Hepatopancreas of Shrimp Metapenaeus ensis / Chapter 1. --- Introduction --- p.20 / Chapter 2. --- Materials and Methods --- p.20 / Chapter 3. --- Results --- p.23 / Chapter 4. --- Discussion --- p.27 / Chapter CHAPTER IV --- Post-embryonic Development of the Alimentary Canalin Shrimp Metapenaeus ensis with emphasis on the Hepatopancreas / Chapter 1. --- Introduction --- p.44 / Chapter 2. --- Materials and Methods --- p.45 / Chapter 3. --- Results --- p.47 / Chapter 4. --- Discussion --- p.54 / Chapter CHAPTER V --- Optimal pH and Temperature for the Assays of Digest ive Enzyme Activities from the Hepatopancreas of Shrimp Metapenaeus ensis / Chapter 1. --- Introduction --- p.89 / Chapter 2. --- Materials and Methods --- p.90 / Chapter 3. --- Results and Discussion --- p.93 / Chapter CHAPTER VI --- Changes in the Hepatopancreas of Shrimp Metapenaeus ensis during the Digestive Cycle / Chapter 1. --- Introduction --- p.100 / Chapter 2. --- Materials and Methods --- p.101 / Chapter 3. --- Results --- p.104 / Chapter 4. --- Discussion --- p.108 / Chapter CHAPTER VII --- Effects of Starvation on the Hepatopancreas in Shrimp Metapenaeus ensis / Chapter 1. --- Introduction --- p.126 / Chapter 2. --- Materials and Methods --- p.127 / Chapter 3. --- Results --- p.130 / Chapter 4. --- Discussion --- p.135 / Chapter CHAPTER VIII --- General Conclusion --- p.160 / REFERENCES --- p.162

by Natalya N. Ovsianico-Koulikowsky. / Thesis (M.Ph.)--Chinese University of Hong Kong, 1987. / Bibliography: leaves 121-134.

by Chung Chi Kong, Arthur. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1992. / Includes bibliographical references (leaves 154-175). / Abstract --- p.i / Acknowledgements --- p.iv / Table of contents --- p.v / List of tables --- p.x / Chapter Chapter 1 --- General introduction --- p.1 / Chapter Chapter 2 --- Literature Review / Chapter 2.1 --- Ovarian maturation of decapod crustaceans / Chapter 2.1.1 --- Female reproductive system --- p.5 / Chapter 2.1.2 --- Ovarian maturation --- p.7 / Chapter 2.1.2.1 --- Previtellogenic stage --- p.11 / Chapter 2.1.2.2 --- Vitellogenic stage --- p.12 / Chapter 2.2 --- Factors affecting ovarian maturation in decapod crustaceans / Chapter 2.2.1 --- Endocrine control --- p.16 / Chapter 2.2.1.1 --- Inhibitory control --- p.17 / Chapter 2.2.1.2 --- Stimulatory control / Chapter 2.2.1.2.1 --- Gonad stimulating hormone (GSH) --- p.18 / Chapter 2.2.1.2.2 --- Vitellogenin stimulating ovarian hormone (VSOH) --- p.19 / Chapter 2.2.1.2.3 --- Ecdysteriods (MH) --- p.20 / Chapter 2.2.1.2.4 --- Juvenoids (JH) --- p.21 / Chapter 2.2.1.2.5 --- Pheromones --- p.22 / Chapter 2.2.1.2.6 --- Vertebrate-like steroids --- p.22 / Chapter 2.2.2 --- Environmental factors --- p.23 / Chapter 2.2.3 --- Nutritional factors --- p.26 / Chapter 2.3 --- The role of lipids during ovarian maturation of decapod crustaceans / Chapter 2.3.1 --- Lipids in decapod crustaceans --- p.29 / Chapter 2.3.2 --- Variation of lipids during ovarian maturation / Chapter 2.3.2.1 --- Introduction --- p.33 / Chapter 2.3.2.2 --- Changes of lipids in the ovary --- p.36 / Chapter 2.3.2.3 --- Changes of lipids in the hepatopancreas --- p.41 / Chapter Chapter 3 --- Variation of lipid composition during ovarian maturation of Penaeus chinensis / Chapter 3.1 --- Introduction --- p.46 / Chapter 3.2 --- Materials and methods / Chapter 3.2.1 --- Experimental animals --- p.46 / Chapter 3.2.2 --- Total lipid extraction and quantification --- p.47 / Chapter 3.2.2.1 --- Total lipid extraction --- p.48 / Chapter 3.2.2.2 --- Quantification of total lipid content --- p.48 / Chapter 3.2.3 --- Separation and quantification of lipid classes --- p.49 / Chapter 3.2.3.1 --- Separation of total lipid classes --- p.49 / Chapter 3.2.3.2 --- Separation of polar lipid classes --- p.50 / Chapter 3.2.3.3 --- Quantification of lipid classes --- p.51 / Chapter 3.2.4 --- Fatty acid analysis / Chapter 3.2.4.1 --- Preparation of fatty acid methyl ester (FAME) --- p.52 / Chapter 3.2.4.2 --- Gas chromatography --- p.53 / Chapter 3.3 --- Results / Chapter 3.3.1 --- Biometric data --- p.54 / Chapter 3.3.2 --- Variation of total lipids --- p.54 / Chapter 3.3.3 --- Variation of lipids in ovary / Chapter 3.3.3.1 --- Neutral lipid classes --- p.55 / Chapter 3.3.3.2 --- Polar lipid classes --- p.56 / Chapter 3.3.3.3 --- Fatty acid composition --- p.56 / Chapter 3.3.4 --- Variation of lipids in hepatopancreas / Chapter 3.3.4.1 --- Neutral lipid classes --- p.57 / Chapter 3.3.4.2 --- Polar lipid classes --- p.58 / Chapter 3.3.4.3 --- Fatty acid composition --- p.58 / Chapter 3.3.5 --- Variation of lipids in muscle / Chapter 3.3.5.1 --- Neutral lipid classes --- p.59 / Chapter 3.3.5.2 --- Polar lipid classes --- p.60 / Chapter 3.3.5.3 --- Fatty acid composition --- p.60 / Chapter 3.4 --- Discussion / Chapter 3.4.1 --- Variation of lipids in ovary during ovarian maturation --- p.88 / Chapter 3.4.2 --- Variation of lipids in hepatopancreas during ovarian maturation --- p.92 / Chapter 3.4.3 --- Variation of lipids in muscle during ovarian maturation --- p.95 / Chapter 3.4.4 --- Mobilization of lipids during ovarian maturation --- p.96 / Chapter Chapter 4 --- Variation of lipid composition during ovarian maturation of Metapenaeus ensis / Chapter 4.1 --- Introduction --- p.101 / Chapter 4.2 --- Materials and methods / Chapter 4.2.1 --- Experimental animals --- p.101 / Chapter 4.2.2 --- Total lipid extraction and quantification --- p.102 / Chapter 4.2.3 --- Separation and quantification of lipid classes --- p.102 / Chapter 4.2.4 --- Fatty acid analysis --- p.103 / Chapter 4.3 --- Results / Chapter 4.3.1 --- Biometric data --- p.104 / Chapter 4.3.2 --- Variation of total lipids --- p.104 / Chapter 4.3.3 --- Variation of lipids in ovary / Chapter 4.3.3.1 --- Neutral lipid classes --- p.105 / Chapter 4.3.3.2 --- Polar lipid classes --- p.105 / Chapter 4.3.3.3 --- Fatty acid composition --- p.106 / Chapter 4.3.4 --- Variation of lipids in hepatopancreas / Chapter 4.3.4.1 --- Neutral lipid classes --- p.107 / Chapter 4.3.4.2 --- Polar lipid classes --- p.108 / Chapter 4.3.4.3 --- Fatty acid composition --- p.109 / Chapter 4.3.5 --- Variation of lipids in muscle / Chapter 4.3.5.1 --- Neutral lipid classes --- p.109 / Chapter 4.3.5.2 --- Polar lipid classes --- p.109 / Chapter 4.3.5.3 --- Fatty acid composition --- p.110 / Chapter 4.4 --- Discussion / Chapter 4.4.1 --- Variation of lipids in ovary during ovarian maturation --- p.138 / Chapter 4.4.2 --- Variation of lipids in hepatopancreas during ovarian maturation --- p.142 / Chapter 4.4.3 --- Variation of lipids in muscle during ovarian maturation --- p.145 / Chapter 4.4.4 --- Mobilization of lipids during ovarian maturation --- p.146 / Chapter Chapter 5 --- General conclusions --- p.151 / References --- p.154

Lo Ting Sze. / Thesis submitted in: December 2002. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (leaves 145-170). / Abstracts in English and Chinese. / Declaration --- p.i / Abstract --- p.ii / Acknowledgements --- p.vii / List of Contents / Contents in Brief --- p.viii / Contents in Detail --- p.ix / List of Figures and Tables / List of Figures --- p.xiii / List of Tables --- p.xvi / Chapter Chapter 1 --- Introduction and literature review General introduction --- p.1 / Chapter 1.1 --- Structural changes in ovary of penaeid shrimp during maturation --- p.3 / Chapter 1.2 --- Biochemical and physiological changes in ovary of penaeid shrimp during maturation --- p.7 / Chapter 1.2.1 --- Variation of protein content --- p.7 / Chapter 1.2.1.1 --- Changes of proteins in ovary of penaeid shrimp during maturation --- p.7 / Chapter 1.2.1.2 --- Biochemical characterization of vitellin --- p.8 / Chapter 1.2.1.3 --- Site of vitellogenin synthesis --- p.10 / Chapter 1.2.2 --- Variation of lipid composition --- p.12 / Chapter 1.2.2.1 --- Role of lipids in ovary of penaeid shrimp --- p.12 / Chapter 1.2.2.2 --- Changes of lipids in ovary of penaeid shrimp during maturation --- p.13 / Chapter 1.2.3 --- Variation of other nutrient contents --- p.16 / Chapter 1.3 --- Endocrine control of ovarian maturation in penaeid shrimp --- p.16 / Chapter 1.3.1 --- Gonad-inhibiting hormone (GIH) --- p.17 / Chapter 1.3.2 --- Crustacean hyperglycemic hormone (CHH) --- p.19 / Chapter 1.3.3 --- X-organ sinus gland complex (XOSG) and CHH neuropeptide family --- p.20 / Chapter 1.3.4 --- Gonad-stimulating hormone (GSH) --- p.22 / Chapter 1.3.5 --- Methyl farnesoate (MF) --- p.23 / Chapter 1.3.6 --- Neurotransmitters --- p.24 / Chapter 1.3.7 --- Other factors --- p.26 / Chapter 1.3.8 --- Androgenic hormone (AH) --- p.27 / Chapter 1.4 --- Ecology and reproductive biology of Metapenaeus ensis --- p.29 / Chapter 1.4.1 --- Identification --- p.29 / Chapter 1.4.2 --- Ecology --- p.29 / Chapter 1.4.3 --- Reproductive biology --- p.30 / Chapter 1.5 --- Objective of research --- p.32 / Chapter Chapter 2 --- "Variation of circulating gonad-inhibiting hormone (GIH) level during ovarian maturation in the shrimp, Metapenaeus ensis" / Chapter 2.1 --- Introduction --- p.34 / Chapter 2.2 --- Materials and methods --- p.35 / Chapter 2.2.1 --- Experimental animals and serum extraction --- p.35 / Chapter 2.2.2 --- Protein content assay --- p.36 / Chapter 2.2.3 --- SDS-polyacrymide gel electrophoresis (SDS-PAGE) --- p.39 / Chapter 2.2.4 --- Western blot --- p.40 / Chapter 2.2.5 --- Purification of IgG from antiserum --- p.42 / Chapter 2.2.6 --- ELISA assay (indirect ELISA) --- p.42 / Chapter 2.3 --- Results --- p.44 / Chapter 2.3.1 --- Experimental animals --- p.44 / Chapter 2.3.2 --- Purification of anti-GIH IgG --- p.46 / Chapter 2.3.3 --- Optimization of indirect ELISA --- p.46 / Chapter 2.3.4 --- Determination of circulating GIH in haemolymph --- p.51 / Chapter 2.4 --- Discussion --- p.55 / Chapter Chapter 3 --- "Identification of genes differentially expressed during ovarian maturation in the shrimp, Metapenaeus ensis" / Chapter 3.1 --- Introduction --- p.60 / Chapter 3.2 --- Materials and methods --- p.61 / Chapter 3.2.1 --- Outline of methodology --- p.61 / Chapter 3.2.2 --- Experimental animals --- p.62 / Chapter 3.2.3 --- Total RNA extraction --- p.62 / Chapter 3.2.4 --- RNA arbitrarily primed PCR (RAP-PCR) --- p.64 / Chapter 3.2.5 --- DNA electrophoresis by agarose gel --- p.68 / Chapter 3.2.6 --- DNA electrophoresis by polyacrylamide gel --- p.68 / Chapter 3.2.7 --- Preparation of DIG-labeled probe from PCR product --- p.69 / Chapter 3.2.8 --- Checking for DIG-labeling yield --- p.69 / Chapter 3.2.9 --- Excision of cDNA library from ovary of Metapenaeus ensis --- p.70 / Chapter 3.2.10 --- PCR screening of insertion sequence --- p.73 / Chapter 3.2.11 --- Dot-blot --- p.75 / Chapter 3.2.12 --- Dot-blot hybridization --- p.76 / Chapter 3.2.13 --- Growth of cell for plasmid preparation --- p.79 / Chapter 3.2.14 --- Permanent culture preparation --- p.80 / Chapter 3.2.15 --- DNA Sequencing --- p.80 / Chapter 3.2.16 --- RNA formaldehyde denaturing gel electrophoresis --- p.82 / Chapter 3.2.17 --- Northern blot --- p.84 / Chapter 3.2.18 --- Northern hybridization --- p.84 / Chapter 3.2.19 --- 5´ة RACE (rapid amplification of cDNA ends) --- p.88 / Chapter 3.3 --- Results --- p.92 / Chapter 3.3.1 --- Experimental animals --- p.92 / Chapter 3.3.2 --- Total RNA extraction --- p.92 / Chapter 3.3.3 --- RNA arbitrarily primed PCR (RAP-PCR) --- p.92 / Chapter 3.3.4 --- Titer of cDNA library from Metapenaeus ensis ovary --- p.95 / Chapter 3.3.5 --- PCR screening of insertion sequence --- p.95 / Chapter 3.3.6 --- Dot-blot hybridization --- p.99 / Chapter 3.3.7 --- DNA sequencing of differentially expressed genes --- p.96 / Chapter 3.3.8 --- Northern analysis --- p.99 / Chapter 3.3.8.1 --- Housekeeping gene - elongation factor 1α --- p.106 / Chapter 3.3.8.2 --- Translationally controlled tumor protein (TCTP) --- p.106 / Chapter 3.3.8.3 --- Cytoskeletal actin --- p.109 / Chapter 3.3.8.4 --- Keratin --- p.109 / Chapter 3.3.8.5 --- Heat shock cognate 70 kD protein (hsc70) --- p.109 / Chapter 3.3.8.6 --- Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) --- p.117 / Chapter 3.3.8.7 --- Arginine kinase --- p.117 / Chapter 3.3.8.8 --- High mobility group 1-like protein (HMGl-like protein) --- p.122 / Chapter 3.3.8.9 --- Nucleoside diphosphate kinase --- p.122 / Chapter 3.4 --- Discussion --- p.122 / Chapter 3.4.1 --- RAP-PCR and dot-blot hybridization --- p.122 / Chapter 3.4.2 --- Functions of differentially expressed genes in ovarian maturation --- p.128 / Chapter 3.4.2.1 --- Translationally controlled tumor protein (TCTP) --- p.128 / Chapter 3.4.2.2 --- Cytoskeletal actin --- p.130 / Chapter 3.4.2.3 --- Keratin --- p.130 / Chapter 3.4.2.4 --- Heat shock cognate 70 kD protein (hsc70) --- p.131 / Chapter 3.4.2.5 --- Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) --- p.134 / Chapter 3.4.2.6 --- Arginine kinase --- p.135 / Chapter 3.4.2.7 --- High mobility group 1-like protein (HMGl-like protein) --- p.136 / Chapter 3.4.2.8 --- Nucleoside diphosphate kinase --- p.137 / Chapter 3.4.3 --- Speculation on the molecular changes in ooctyes during ovarian maturation --- p.139 / Chapter Chapter 4 --- General conclusion --- p.142 / References --- p.145

Mak Man Ting. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1997. / Includes bibliographical references (leaves 116-125). / ABSTRACT --- p.i / ACKNOWLEDGMENTS --- p.iv / TABLE OF CONTENTS --- p.v / LIST OF TABLES --- p.ix / LIST OF FIGURES --- p.x / Chapter CHAPTER1 --- INTRODUCTION --- p.1 / Chapter CHAPTER2 --- LITERATURE REVIEW / Chapter 2.1 --- General Review on Crustacean Circulatory System --- p.3 / Chapter 2.1.1 --- General anatomy and function --- p.3 / Chapter 2.1.2 --- Control mechanisms of cardiac function --- p.5 / Chapter 2.1.2.1 --- Intrinsic control --- p.6 / Chapter 2.1.2.2 --- Extrinsic control --- p.6 / Chapter 2.1.3 --- Ontogenic changes in cardiac function --- p.8 / Chapter 2.1.3.1 --- Change in heart rate during development --- p.8 / Chapter 2.1.3.2 --- Change from myogenic heart to neurogenic heart --- p.9 / Chapter 2.2 --- Effect of Temperature on Crustacean Cardiac Function --- p.10 / Chapter 2.2.1 --- General effect of temperature on crustaceans --- p.10 / Chapter 2.2.2 --- Effect of temperature on heart rate --- p.11 / Chapter 2.2.3 --- "Inter-relationship between heart rate, stroke volume and cardiac output" --- p.14 / Chapter 2.2.4 --- Control mechanisms on crustacean cardiac function in response to temperature change --- p.15 / Chapter 2.3 --- Effect of Salinity on Crustacean Cardiac Function --- p.18 / Chapter 2.3.1 --- General effect of salinity on crustaceans --- p.18 / Chapter 2.3.2 --- Effect of salinity on heart rate --- p.21 / Chapter 2.3.3 --- Effect of salinity on hemolymph flow distribution --- p.25 / Chapter CHAPTER3 --- CHANGES IN HEART RATE DURING DEVELOPMENT / Chapter 3.1 --- Introduction --- p.27 / Chapter 3.2 --- Materials and Methods --- p.29 / Chapter 3.3 --- Results --- p.31 / Chapter 3.4 --- Discussion --- p.34 / Chapter CHATPER4 --- EFFECT OF SALINITY ON HEART RATE OF METAPENAEUS ENSIS / Chapter 4.1 --- Introduction --- p.43 / Chapter 4.2 --- Materials and Methods --- p.45 / Chapter 4.3 --- Results --- p.48 / Chapter 4.4 --- Discussion --- p.51 / Chapter CHATPER5 --- EFFECT OF TEMPERATURE ON HEART RATE OF METAPENAEUS ENSIS / Chapter 5.1 --- Introduction --- p.58 / Chapter 5.2 --- Materials and Methods --- p.59 / Chapter 5.3 --- Results --- p.62 / Chapter 5.4 --- Discussion --- p.77 / Chapter CHAPTER6 --- DEVELOPMENT OF HEART INNERVATION IN METAPENAEUS ENSIS / Chapter 6.1 --- Introduction --- p.86 / Chapter 6.2 --- Materials and Methods --- p.87 / Chapter 6.3 --- Results --- p.89 / Chapter 6.4 --- Discussion --- p.109 / Chapter CHAPTER7 --- CONCLUSIONS --- p.112 / REFERENCES --- p.116

Cui, Ju. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2007. / Includes bibliographical references (leaves 110-136). / Abstracts in English and Chinese. / Declaration --- p.i / Abstract --- p.ii / Acknowledgements --- p.viii / Table of Contents --- p.ix / List of Tables and Figures --- p.xi / List of Abbreviations --- p.xiii / Chapter 1 General introduction --- p.1 / Chapter 2 Literature review --- p.4 / Chapter 2.1 --- Introduction --- p.4 / Chapter 2.2 --- Structural changes in ovary and thelycum of penaeid shrimp during maturaiton --- p.4 / Chapter 2.3 --- Biochemical changes in ovary of penaeid shrimp during maturation --- p.7 / Chapter 2.4 --- Endocrine control of ovarian maturation in penaeid shrimp --- p.9 / Chapter 2.4.1 --- Peptides --- p.9 / Chapter 2.4.2 --- Steroids --- p.17 / Chapter 2.4.3 --- Terpenoids --- p.19 / Chapter 2.4.4 --- Biogenic amines --- p.21 / Chapter 2.6 --- Reproductive biology of the shrimp Metapenaeus ensis --- p.23 / Chapter 2.6 --- Proteomics --- p.27 / Chapter Chapter 3 --- Identification of proteins differentially expressed during ovarian maturation in the shrimp Metapenaeus ensis --- p.28 / Chapter 3.1 --- Introduction --- p.28 / Chapter 3.2 --- Materials and methods --- p.30 / Chapter 3.2.1 --- Animals --- p.30 / Chapter 3.2.2 --- Histological observation --- p.30 / Chapter 3.2.3 --- Data analysis --- p.31 / Chapter 3.2.4 --- Two-dimensional gel electrophoresis --- p.31 / Chapter 3.2.5 --- Protein isolation and identification by MALDI-TOF MS/MS --- p.35 / Chapter 3.3 --- Results --- p.37 / Chapter 3.3.1 --- General ovarian histology --- p.37 / Chapter 3.3.2 --- Morphometric analysis --- p.41 / Chapter 3.3.3 --- Comparison of proteomic patterns of shrimp ovaries and image analysis --- p.43 / Chapter 3.3.4 --- Identification of differentially expressed proteins in shrimp ovaries by MALDI-TOF MS/MS analysis --- p.48 / Chapter 3.4 --- Discussion --- p.61 / Chapter 3.4.1 --- Identification of proteins by MALDI-TOF MS/MS analysis --- p.61 / Chapter 3.4.2 --- Potential functions of the identified ditterentially expressed proteins in shrimp reproduction --- p.62 / Chapter Chapter 4 --- Characterization of cellular retinoic acid binding protein (CRABP) and retinoic X receptor (RXR) in the shrimp Metapenaeus ensis --- p.69 / Chapter 4.1 --- Introduction --- p.69 / Chapter 4.2 --- Materials and methods --- p.72 / Chapter 4.2.1 --- Experimental animals --- p.72 / Chapter 4.2.2 --- Preparation of total RNA --- p.72 / Chapter 4.2.3 --- Rapid Amplification of 5' and 3' cDNA Ends (RACE) --- p.73 / Chapter 4.2.4 --- Subcloning --- p.75 / Chapter 4.2.5 --- Sequencing --- p.78 / Chapter 4.2.6 --- Phylogenetic analysis --- p.78 / Chapter 4.2.7 --- Reverse transcription (RT) --- p.79 / Chapter 4.2.8 --- Semi-quantitative RT-PCR --- p.79 / Chapter 4.2.9 --- Real-Time RT-PCR --- p.80 / Chapter 4.2.10 --- In situ hybridization --- p.81 / Chapter 4.2.11 --- Tissue culture and in vitro ovary explant assay --- p.83 / Chapter 4.2.12 --- Statistical analysis --- p.84 / Chapter 4.3 --- Results --- p.84 / Chapter 4.3.1 --- Full-length RXR cDNA derivation --- p.84 / Chapter 4.3.2 --- Sequence comparisons and phylogenetic analyses --- p.86 / Chapter 4.3.3 --- Spatiotemporal expression profiles of MeCRABP and MeRXR mRNA in female shrimp --- p.92 / Chapter 4.3.4 --- Effect of exogenous retinoic acids on the expression of MeCRABP and MeRXR in shrimp ovaries --- p.96 / Chapter 4.4 --- Discussion --- p.101 / Chapter 4.4.1 --- Cloning and characterization of the M. ensis RXR --- p.101 / Chapter 4.4.2 --- Developmental expression of CRABP and RXR in shrimp ovary --- p.102 / Chapter 4.4.3 --- Effects of exogenous retinoic acids --- p.104 / Chapter Chapter 5 --- General conclusion --- p.106 / References --- p.110

by Patrick Kah Kin Leong. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1991. / Includes bibliographical references. / ABSTRACT --- p.i / ACKNOWLEDGEMENTS --- p.iii / TABLE OF CONTENTS --- p.iv / LIST OF TABLES --- p.vii / LIST OF FIGURES --- p.viii / LIST OF PLATES --- p.xi / LIST OF ABBREVIATIONS --- p.xii / Chapter CHAPTER1 --- INTRODUCTION --- p.1 / Chapter CHAPTER2 --- LITERATURE REVIEW / Chapter 2.1 --- Biology of Metapenaeus ensis --- p.4 / Chapter 2.2 --- Definitions in the measurements of zooplankton feeding rate --- p.5 / Chapter 2.3 --- Zooplankton feeding rates and food concentration --- p.6 / Chapter 2.4 --- Other factors affecting feeding behaviour --- p.11 / Chapter 2.5 --- Feeding patterns of the early life history stages in various crustaceans --- p.14 / Chapter 2.6 --- Selective feeding --- p.17 / Chapter CHAPTER3 --- LARVAL DEVELOPMENT OF Metapenaeus ensis / Chapter 3.1 --- Introduction --- p.20 / Chapter 3.2 --- Materials and Methods --- p.22 / Chapter 3.3 --- Results --- p.25 / Chapter 3.4 --- Discussion --- p.52 / Chapter CHAPTER4 --- THE GRAZING BEHAVIOUR OF EARLY LIFE HISTORY STAGES OF Metapenaeus ensis / Chapter 4.1 --- Introduction --- p.86 / Chapter 4.2 --- Materials and Methods --- p.87 / Chapter 4.3 --- Results --- p.90 / Chapter 4.4 --- Discussion --- p.94 / Chapter CHAPTER5 --- THE SELECTIVE FEEDING BEHAVIOUR OF EARLY LIFE HISTORY STAGES OF Metapenaeus ensis / Chapter 5.1 --- Introduction --- p.109 / Chapter 5.2 --- Materials and Methods --- p.110 / Chapter 5.3 --- Results --- p.115 / Chapter 5.4 --- Discussion --- p.117 / Chapter CHAPTER6 --- CONCLUSION --- p.133 / REFERENCES --- p.136

Mak Wai Yan. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2006. / Includes bibliographical references (leaves 95-115). / Abstracts in English and Chinese. / Declaration --- p.i / Abstract --- p.ii / Acknowledgements --- p.viii / Table of Contents --- p.ix / List of Tables --- p.xiii / List of Figures --- p.xiv / List of Abbreviations --- p.xvi / Chapter Chapter 1 --- Introduction and Literature Review / Chapter 1.1 --- Introduction --- p.1 / Chapter 1.2 --- Endocrine control of reproduction in crustaceans --- p.2 / Chapter 1.3 --- The X-organ sinus gland (XOSG) complex --- p.3 / Chapter 1.3.1 --- CHH/MIH/GIH family of neuropeptides --- p.4 / Chapter 1.3.2 --- Gonad inhibiting hormone (GIH) --- p.6 / Chapter 1.3.3 --- Molt inhibiting hormone (MIH) --- p.7 / Chapter 1.3.4 --- Crustaceans hyperglycemic hormone (CHH) --- p.9 / Chapter 1.3.5 --- The chormatophorotropins (RPCH and PDH neuropeptide family) --- p.10 / Chapter 1.4 --- Other mechanisms of reproduction control --- p.11 / Chapter 1.4.1 --- Methyl farnesoate (MF) and mandibular organ inhibiting hormone (MOIH) --- p.11 / Chapter 1.4.2 --- Gonad stimulating hormone (GSH) --- p.13 / Chapter 1.4.3 --- Serotonin (5HT) --- p.13 / Chapter 1.4.4 --- Dopamine --- p.14 / Chapter 1.4.5 --- Enkephalin (ENK) --- p.15 / Chapter 1.4.6 --- 17β-Estradiol --- p.16 / Chapter 1.4.7 --- Progesterone --- p.17 / Chapter 1.5 --- Androgenic hormone (AH) --- p.18 / Chapter 1.6 --- Objective and methodology of present research --- p.19 / Chapter 1.7 --- Reproductive biology of the shrimp Metapenaeus ensis --- p.20 / Chapter Chapter 2 --- Materials and Methods / Chapter 2.1 --- Introduction --- p.26 / Chapter 2.2 --- Extraction of eyestalk RNA from the shrimp --- p.27 / Chapter 2.3 --- Construction of the eyestalk cDNA library --- p.28 / Chapter 2.3.1 --- First-strand cDNA synthesis --- p.28 / Chapter 2.3.2 --- cDNA amplification by LD PCR --- p.29 / Chapter 2.3.3 --- Proteinase K digestion --- p.29 / Chapter 2.3.4 --- Sfi I digestion --- p.30 / Chapter 2.3.5 --- cDNA size fractionation --- p.30 / Chapter 2.3.6 --- Ligation of cDNA to λ TriplEx2 Vector --- p.31 / Chapter 2.3.7 --- Packaging --- p.31 / Chapter 2.3.8 --- Titering the unamplified library --- p.32 / Chapter 2.3.9 --- Library amplification --- p.33 / Chapter 2.3.10 --- Titering the amplified library --- p.34 / Chapter 2.4 --- Mass Excision of the eyestalk cDNA library --- p.34 / Chapter 2.5 --- PCR screening of inserted sequence --- p.35 / Chapter 2.6 --- RNA arbitrarily primed polymerase chain reaction (RAP-PCR) --- p.35 / Chapter 2.7 --- Dot blot hybridization --- p.37 / Chapter 2.7.1 --- Probe Labelling --- p.37 / Chapter 2.7.2 --- Dotting of membrane --- p.38 / Chapter 2.7.3 --- Hybridization --- p.38 / Chapter 2.7.4 --- Washing and chemiluminescent detection --- p.39 / Chapter 2.7.5 --- Probe stripping for re-hybridization --- p.40 / Chapter 2.8 --- Sequencing --- p.40 / Chapter 2.9 --- BLAST search --- p.41 / Chapter 2.10 --- Northern blot analysis --- p.41 / Chapter 2.10.1 --- Probe labelling --- p.41 / Chapter 2.10.2 --- RNA formaldehyde denaturing gel electrophoresis --- p.41 / Chapter 2.10.3 --- Northern blot --- p.42 / Chapter 2.10.4 --- Pre-hybridization --- p.43 / Chapter 2.10.5 --- Hybridization --- p.43 / Chapter 2.10.6 --- Washing and chemiluminescent detection --- p.43 / Chapter 2.11 --- Real-time RT-PCR --- p.44 / Chapter 2.11.1 --- DNaseI treatment --- p.44 / Chapter 2.11.2 --- First strand synthesis --- p.44 / Chapter 2.11.3 --- Primer design and verification --- p.45 / Chapter 2.11.4 --- Real-time PCR --- p.45 / Chapter 2.11.5 --- Statistical analysis --- p.45 / Chapter Chapter 3 --- Results / Chapter 3.1 --- Experimental animals --- p.50 / Chapter 3.2 --- Total RNA extraction --- p.50 / Chapter 3.3 --- Library construction --- p.50 / Chapter 3.4 --- PCR screening of inserted sequences --- p.50 / Chapter 3.5 --- RNA arbitrarily primed polymerase chain reaction (RAP-PCR) --- p.51 / Chapter 3.6 --- Dot blot hybridization --- p.51 / Chapter 3.7 --- Sequencing and BLAST search --- p.52 / Chapter 3.8 --- Northern blot analysis --- p.53 / Chapter 3.8.1 --- Housekeeping gene - elongation factor la --- p.53 / Chapter 3.8.2 --- Insulin-like growth factor binding protein (IGFBP) --- p.54 / Chapter 3.8.3 --- Arrestin --- p.54 / Chapter 3.8.4 --- Opsin --- p.54 / Chapter 3.9 --- Real-time RT-PCR --- p.55 / Chapter 3.9.1 --- β-Actin --- p.55 / Chapter 3.9.2 --- Farnesoic acid O-methyltranferase (FAMeT) --- p.56 / Chapter Chapter 4 --- Discussion / Chapter 4.1 --- Application of RAP-PCR and dot blot analysis in identifying differential expressed genes --- p.79 / Chapter 4.1.1 --- Abundant genes --- p.79 / Chapter 4.1.2 --- Appearance of 16S rRNA in eyestalk cDNA library of M. ensis --- p.80 / Chapter 4.1.3 --- False positive --- p.80 / Chapter 4.2 --- Investigation on common housekeeping genes --- p.81 / Chapter 4.3 --- Potential functions of identified differential expressed genes in reproduction of shrimp --- p.82 / Chapter 4.3.1 --- Arrestin --- p.84 / Chapter 4.3.2 --- Opsin --- p.86 / Chapter 4.3.3 --- Insulin-like growth factor binding protein (IGFBP) --- p.88 / Chapter 4.3.4 --- β-Actin --- p.89 / Chapter 4.4 --- Investigation for farnesoic acid O-methyltransferase (FAMeT) in shrimp --- p.89 / Chapter Chapter 5 --- General Conclusion --- p.91 / References --- p.95 / Appendix --- p.116

by Tam Yan Kit. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1992. / Includes bibliographical references (leaves 102-114). / Abstract --- p.1 / Acknowledgement --- p.3 / Table of Contents --- p.5 / List of Tables --- p.7 / List of Figures --- p.8 / List of Plates --- p.8 / Chapter Chapter1 --- Introduction --- p.9 / Chapter Chapter2 --- Literature Review / Chapter 2.1 --- The study of enzyme polymorphism --- p.11 / Chapter 2.2 --- The zymogram technique --- p.20 / Chapter 2.3 --- Molecular approaches to systematic studies --- p.24 / Chapter 2.4 --- Genetic variation and systematic studies of decapod Crustacea --- p.29 / Chapter 2.5 --- Taxonomy of Penaeus and Metapenaeus --- p.33 / Chapter Chapter3 --- Materials and Methods / Chapter 3.1 --- Sample collection and storage --- p.43 / Chapter 3.2 --- Extract preparation --- p.44 / Chapter 3.3 --- Starch gel preparation --- p.45 / Chapter 3.4 --- Starch gel electrophoresis --- p.46 / Chapter 3.5 --- Gel slicing --- p.47 / Chapter 3.6 --- Enzyme staining --- p.47 / Chapter 3.7 --- Data collection --- p.48 / Chapter 3.8 --- Data analysis --- p.49 / Chapter Chapter4 --- Results / Chapter 4.1 --- Genetic interpretation of zymograms --- p.65 / Chapter 4.2 --- Conformity to Hardy-Weinberg equilibrium distribution --- p.67 / Chapter 4.3 --- Level of genetic variability --- p.67 / Chapter 4.4 --- Interspecific and intergeneric genetic differentiation --- p.68 / Chapter Chapter5 --- Discussion / Chapter 5.1 --- Enzyme polymorphism in Penaeus and Metapenaeus --- p.84 / Chapter 5.2 --- Level of genetic variability --- p.85 / Chapter 5.3 --- Phylogenetic relationships --- p.91 / Chapter 5.4 --- Biochemical and molecular systematic studies of Penaeus and Metapenaeus --- p.96 / Chapter Chapter6 --- General Conclusions --- p.99 / References --- p.102

Tang Chi-Yan. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2005. / Includes bibliographical references (leaves 89-112). / Abstracts in English and Chinese. / Abstract --- p.i / Acknowledgements --- p.vi / Table of contents --- p.viii / List of Tables --- p.xi / List of Figures --- p.xii / List of Abbreviations --- p.xiv / Chapter Chapter 1. --- General introduction --- p.1 / Chapter Chapter 2. --- Literature review --- p.4 / Chapter 2.1 --- History and prevalence of food allergy --- p.4 / Chapter 2.2 --- Mechanism and clinical symptoms of food allergy --- p.6 / Chapter 2.3 --- Tropomyosin as a major shellfish allergen --- p.13 / Chapter 2.4 --- Use of animal model in the studies of food allergy --- p.22 / Chapter 2.5 --- Future approaches for treatment of food allergy --- p.27 / Chapter Chapter 3. --- Cloning and expression of recombinant tropomyosin --- p.30 / Chapter 3.1 --- Introduction --- p.30 / Chapter 3.2 --- Materials and Methods --- p.31 / Chapter 3.2.1 --- Design of PCR primers for amplification of tropomyosin gene --- p.31 / Chapter 3.2.2 --- Cloning of PCR-amplified cDNA into vector --- p.32 / Chapter 3.2.3 --- Transformation of competent E. coli Ml5 cells --- p.34 / Chapter 3.2.4 --- Confirmation of DNA sequence of the cloned vector --- p.34 / Chapter 3.2.5 --- Induction of the recombinant protein --- p.35 / Chapter 3.2.6 --- Purification and storage of the recombinant protein under native condition --- p.36 / Chapter 3.2.7 --- Concentration measurement and storage of the recombinant protein --- p.37 / Chapter 3.2.8 --- Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) --- p.38 / Chapter 3.2.9 --- Regeneration of the Ni-NTA column --- p.40 / Chapter 3.3 --- Results and discussion --- p.42 / Chapter 3.3.1 --- DNA sequence of the cloned vector --- p.42 / Chapter 3.3.2 --- Expression of the recombinant protein --- p.42 / Chapter 3.3.3 --- Sodium dodecyl sulfate polyacrylamide gel eletrophoresis (SDS-PAGE) --- p.43 / Chapter Chapter 4. --- Induction of hypersensitive response to shrimp tropomyosin in mice --- p.47 / Chapter 4.1 --- Introduction --- p.47 / Chapter 4.2 --- Materials and methods --- p.52 / Chapter 4.2.1 --- Mice and reagents --- p.52 / Chapter 4.2.2 --- Animal sensitization and challenge --- p.53 / Chapter 4.2.3 --- Morphological and behavioral changes --- p.54 / Chapter 4.2.4 --- Tropomyosin-specific IgE level --- p.55 / Chapter 4.2.5 --- Passive cutaneous anaphylaxis (PCA) reaction --- p.56 / Chapter 4.2.6 --- Tropomyosin-specific cellular proliferation level of splenocytes --- p.56 / Chapter 4.2.7 --- Cytokine profiles of splenoctyes --- p.58 / Chapter 4.2.8 --- Histological examination of small intestine --- p.59 / Chapter 4.2.9 --- Statistical analysis --- p.59 / Chapter 4.3 --- Results --- p.63 / Chapter 4.3.1 --- Morphological and behavioral changes after challenge --- p.63 / Chapter 4.3.2 --- Tropomyosin-specific IgE level --- p.63 / Chapter 4.3.3 --- Passive cutaneous anaphylaxis (PCA) --- p.64 / Chapter 4.3.4 --- Tropomyosin-specific cellular proliferation level of splenocytes --- p.68 / Chapter 4.3.5 --- Cytokine profiles of splenocytes --- p.70 / Chapter 4.3.6 --- Histology of small intestines --- p.76 / Chapter 4.4 --- Discussion --- p.79 / Chapter Chapter 5. --- General conclusion --- p.88 / References --- p.89