The present study is aimed at examining the ability of Pichia pastoris to utilize acetate and amino acids as the sole sources of carbon. We demonstrate that the zinc finger transcription factor Mxr1p, which is a positive regulator of methanol metabolism, is also required for the growth of P. pastoris in media containing acetate or amino acids as the sole source of carbon. We have identified the target genes of Mxr1p in cells cultured in media containing acetate or amino acids as the sole carbon source. We conclude that Mxr1p is a global regulator of multiple metabolic pathways in P. pastoris.
| Identifer | oai:union.ndltd.org:IISc/oai:etd.ncsi.iisc.ernet.in:2005/3212 |
| Date | January 2016 |
| Creators | Sahu, Umakant |
| Contributors | Rangarajan, P N |
| Source Sets | India Institute of Science |
| Language | en_US |
| Detected Language | English |
| Type | Thesis |
| Relation | G28356 |
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