nÃo hà / As infecÃÃes entÃricas causam cerca de 2,5 milhÃes de mortes ao ano. A EAEC està associada à causa de doenÃas diarrÃicas persistentes. Este estudo analisou in vitro (IEC-6, Caco-2 e HEp-2), o papel dos micronutrientes glutamina, alanil-glutamina, Ã-caroteno, zinco, e dos leites de cabra transgÃnico com lisozima humana e controle nos ensaios de proliferaÃÃo, migraÃÃo, viabilidade, apoptose, necrose celular, adesÃo bacteriana em resposta à infecÃÃo causada pela cepa de EAEC-042 na concentraÃÃo de 2,5 x 105 UFC/mL. A cepa bacteriana de EAEC-042 mostrou reduÃÃo significativa na migraÃÃo (p<0,001) e na viabilidade celular (p<0,001) e esta aumentou a apoptose (p<0,001) e necrose (p<0,001) em resposta a lesÃo ao epitÃlio intestinal. Foi observado que os micronutrientes na presenÃa da bactÃria reduziram significativamente a apoptose e necrose ocasionados por esta, bem como reduziram significativamente a adesÃo bacteriana, alÃm de aumentar a migraÃÃo celular. Os leites controle e transgÃnico apresentaram reduÃÃo significativa da adesÃo bacteriana (p<0,001), independente da presenÃa da camada de gordura, alÃm de reduzirem significativamente a apoptose (p<0,001) e a necrose (p<0,001) ocasionadas pela EAEC-042. A anÃlise qualitativa de aderÃncia celular, considerada padrÃo ouro, mostrou reduÃÃo na aderÃncia bacteriana quando associados aos micronutrientes, comparados ao controle com EAEC-042. Nota-se a quase ausÃncia de aderÃncia em ambos os leites. Este estudo mostra a importÃncia dos micronutrientes e leite de cabra transgÃnico ou nÃo, sobre a proteÃÃo epitelial intestinal nas agressÃes bacterianas. / The enteric infections cause 2.5 million deaths each year. The Enteroaggregative Escherichia coli (EAEC) is associated with persistent cause of diarrheal diseases. This study examined in vitro (IEC-6, Caco-2 and HEp-2 cells) the role of the micronutrients glutamine (Glu), alanyl-glutamine (Ala-Glu), beta-carotene (Ã-Carot), zinc (Zn), and the milk of transgenic goats containning human lysozyme (M-Lyso) and their respective controls (Ctrle) in the following assays: proliferation, migration, viability, apoptosis, cell necrosis, and bacterial adhesion in response to infection caused by the EAEC-042 bacterial strain at a concentration of 2.5 x 105 CFU/mL. The effect of infection by EAEC-042 bacterial strain was evidenced by significant reduction in migration (p <0.001) and cellular viability (p <0.001); also increased apoptosis (p <0.001) and necrosis (p <0.001) in response to damage to the intestinal epithelium. It was observed that the micronutrients in the presence of bacteria significantly reduced apoptosis and necrosis caused by EAEC-042, as well as significantly reduced bacterial adhesion and increases cell migration. The control and transgenic milk abolished bacterial adhesion (p <0.001), independent of milk fat, and significantly reduce apoptosis (p <0.001) and necrosis (p <0.001) caused by EAEC-042. The qualitative analysis of EAEC adherence, considered as gold standard method, showed a reduction in bacterial adherence associated with intervention with micronutrients when compared with the EAEC-042 infection control. In conclusion, our study demonstrates the importance of intervention with micronutrients and milk (transgenic or not) in protecting the intestinal epithelial challenged by bacterial aggression.
| Identifer | oai:union.ndltd.org:IBICT/oai:www.teses.ufc.br:7545 |
| Date | 29 July 2011 |
| Creators | Eunice Bobà de Carvalho |
| Contributors | Aldo Ãngelo Moreira Lima, Reinaldo Barreto OriÃ, Alexandre Havt Bindà |
| Publisher | Universidade Federal do CearÃ, Programa de PÃs-GraduaÃÃo em CiÃncias MÃdicas, UFC, BR |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFC, instname:Universidade Federal do Ceará, instacron:UFC |
| Rights | info:eu-repo/semantics/openAccess |
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