The salamander is the only tetrapod that regenerates complex body structures throughout life. Deciphering the underlying molecular
processes of regeneration is fundamental for regenerative medicine and developmental biology, but the model organism had limited tools
for molecular analysis. We describe a comprehensive set of germline transgenic strains in the laboratory-bred salamander Ambystoma
mexicanum (axolotl) that open up the cellular and molecular genetic dissection of regeneration.We demonstrate tissue-dependent control
of gene expression in nerve, Schwann cells, oligodendrocytes, muscle, epidermis, and cartilage. Furthermore, we demonstrate the use
of tamoxifen-induced Cre/loxP-mediated recombination to indelibly mark different cell types. Finally, we inducibly overexpress the cellcycle
inhibitor p16INK4a, which negatively regulates spinal cord regeneration. These tissue-specific germline axolotl lines and tightly
inducible Cre drivers and LoxP reporter lines render this classical regeneration model molecularly accessible.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:28890 |
| Date | 27 October 2015 |
| Creators | Tanaka, Elly M., Khattak, Shahryar, Schuez, Maritta, Richter, Tobias, Knapp, Dunja, Haigo, Saori L., Sandoval-Guzmán, Tatiana, Hradlikova, Kristyna, Duemmler, Annett, Kerney, Ryan |
| Publisher | Elsevier |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 2213-6711, 10.1016/j.stemcr.2013.03.002 |
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