Introduction Breast cancer is the most common cancer among women in Sweden and world-wide. Immunohistochemical (IHC) expression of oestrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2) and Ki-67 is routinely performed for diagnostic and prognostic purposes. Based on these biomarkers tumours are divided into subtypes: Luminal A, Luminal B, HER2-enriched and triple negative breast cancer (TNBC). There is evidence that tumour associated macrophages (TAMs) have a crucial role in tumour development. To identify TAMs the antibody CD163 can be used in immunohistochemical staining. Aim The aim of the study was to investigate differences in density of CD163+ macrophages among different subtypes of breast cancer. Material and method Formalin-fixed paraffin-embedded (FFPE) breast cancer tissue from the Biobank of Region Värmland was used. Tissue was de-identified before analysis. Groups were formed based on immunohistochemical expression of ER, PR and HER2, with the exclusion of the Luminal B group. Tissue was stained with CD163. Analysis was performed semi-quantitatively. The border between tumour and healthy tissue was assessed as well as the intra-tumoral tissue. Results At the tumour border, density of CD163+ macrophages was found to be lower in the Luminal group compared to both the HER2-enriched and TNBC.Within intra-tumoral tissue, the density of CD163+ macrophages was found to be lower in the Luminal compared to the HER2-enriched group. Conclusion The density of CD163+ macrophages is higher in HER-enriched and TNBC tumours compared to Luminal A breast cancer tumours.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:oru-103455 |
| Date | January 2022 |
| Creators | Andersson, Julia |
| Publisher | Örebro universitet, Institutionen för medicinska vetenskaper |
| Source Sets | DiVA Archive at Upsalla University |
| Language | English |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
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