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Previous issue date: 2014-03-28 / Injury of peripheral nerves of accidents at work or home are common carriers and scallfolds of biodegradable polymers with temporary structural function to assist in the regeneration of damaged living tissue are being explored. Conducting polymers such as polypyrrole (PPy), have also been explored because of biocompatibility to various cell types and ease of synthesis. Artificial Nerve Guidance Conduits with multifunctional growth factors (GF) have been studied to improve the regeneration of injured peripheral nerves. The main objective is to prepare polymer composites of poly (lactic - co - glycolic acid) - (PLGA) with PPy nanofibers able to act as a conduit in peripheral nerve regeneration. PPy nanofibers were synthesized via chemical oxidative polymerization with different dopants. Films PLGA / nanofiber PPy / tacrolimus (FK506) were prepared by sandwich solvent casting method. The PPy nanofibers were characterized by FTIR, SEM, electrical impedance spectroscopy and TGA. In vitro degradation of PLGA/PPy composite films was also evaluated. PPy nanofibers of electrically condutive 8.10-4 S / cm were obtained with dopant p-toluenesulfonic acid (PTSA) in the ratio [dop]: [Py] = 4 and 0 ? C. Films PLGA / PPy-nanofibers exhibit irregular surface morphology with voids that can serve as framewoks for cell growth guided. The degradation of PLGA / PPy films did not alter the pH of the buffer solution, an increase of thickness and mass loss was in the range of 7-21% until 28 days of degradation reviews. The addition of nanofibers favored the process of mass loss of about 4% for the PLGA / PPy thicker (≥ 0.6g of PLGA) films and 6% for thinner films (0.2 g PLGA) until 28 days degradation. / Les?es dos nervos perif?ricos por acidentes de trabalho ou dom?stico s?o comuns e suportes de pol?meros biodegrad?veis com fun??o estrutural tempor?ria para auxiliarem na regenera??o de tecidos vivos lesados v?m sendo explorados. Pol?meros condutores, como o polipirrol (PPy), tamb?m t?m sido investigados devido facilidade de s?ntese e biocompatibilidade.Condutos Artificiais de Orienta??o Neural multifuncional com fatores de crescimento (FC) v?m sendo estudados para aperfei?oar a regenera??o de feridas em nervos perif?ricos. O objetivo principal deste trabalho ? preparar comp?sitos polim?ricos de poli (?cido l?ctico-co-?cido glic?lico) - (PLGA) com nanofibras de PPy capazes de atuarem como conduto guia na regenera??o de nervos perif?ricos. Nanofibras de PPy foram sintetizadas via polimeriza??o qu?mica oxidativa com diferentes agentes dopantes. Filmes de PLGA/nanofibras PPy/Tacromilus (FK506) foram preparados pelo m?todo do sandu?che por evapora??o do solvente. As nanofibras de PPy foram caracterizadas por FTIR, MEV, espectroscopia de imped?ncia el?trica e TGA. Tamb?m foi avaliada a degrada??o in vitro dos filmes comp?sitos de PLGA/PPy. As nanofibras de PPy com condutividade el?trica de 8.10-4 S/cm foram obtidas com dopante ?cido p-toluenosulf?nico (APTS) na raz?o [dop]:[Py] = 4 e na temperatura de 0?C. Filmes de PLGA/PPy-nanofibras apresentam morfologia superficial irregular fibrosa com poros aleat?rios que podem servir de arcabou?os para o crescimento celular. A degrada??o dos filmes PLGA/PPy n?o alteraram o pH do meio, houve aumento de espessuras e as perdas de massas ficaram na faixa de 7-21 % at? os 28 dias de degrada??o avaliados. A adi??o das nanofibras favoreceu o processo de perda de massa cerca de 4% para os filmes PLGA/PPy mais espessos (≥ 0,6g de PLGA) e 6% para filmes mais finos (0,2 g de PLGA) at? os 28 dias de degrada??o.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/3268 |
| Date | 28 March 2014 |
| Creators | Valente, Cristhiane Alvim |
| Contributors | Basso, Nara Regina de Souza |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Engenharia e Tecnologia de Materiais, PUCRS, BR, Faculdade de Engenharia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -7432719344215120122, 500, 600, -655770572761439785 |
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