Sequence analysis of tRNA 7 ser from Drosophila melanogaster was
carried out, primarily by the formamide degradation and post-labeling method
of Stanley and Vassilenko. Preliminary analysis was on the terminal
nucleoside-5', 3'-bis [51 - ³²P] phosphates of electrophoretically separated
[51 - ³²P] ribooligonucleotides, identifying the [³² P]-nucleotides by
chromatography on PEI-cel1ulose plates. Further analysis of possible
modified nucleotides was performed by thin layer chromatography of [5' -³²P] nucleoside phosphates derived by nuclease P-j digestion from [51 -
³²P] oligomers. The partial sequence generated in this fashion was
supplemented by ladder gel sequence analysis of [51 - P]tRNA ser , and to a limited extent by two dimensional homochromatography. In this way, a sequence was obtained that is complete except for part of the aminoacyl stem and the 5'-end of the extra arm. The data are consistent with a sequence for tRNAser of pGCAGmUUGUGGCac4CGAGCGmGDDAAGGCXUCUGA— m3CUI GAi 6AAujCAGAUmUCCCUm3CUGGGAGm5CGUAGGTijjCGm1 AAUCCUACCGACUGCNCCA. / Medicine, Faculty of / Biochemistry and Molecular Biology, Department of / Unknown
| Identifer | oai:union.ndltd.org:UBC/oai:circle.library.ubc.ca:2429/21567 |
| Date | January 1979 |
| Creators | Cribbs, David Lamar |
| Source Sets | University of British Columbia |
| Language | English |
| Detected Language | English |
| Type | Text, Thesis/Dissertation |
| Rights | For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use. |
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