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Cloning and analysis of promoter regulating the expression of a purple acid phosphatase.

Zhang Siyi. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2001. / Includes bibliographical references (leaves 97-109). / Abstracts in English and Chinese. / Acknowledgements --- p.i / Abstract --- p.iii / List of Tables --- p.vii / List of Figures --- p.viii / List of Abbreviations --- p.x / Chapter Chapter 1: --- General Introduction --- p.1 / Chapter Chapter 2: --- Literature Review --- p.3 / Chapter 2.1 --- Phosphorus and higher plants --- p.3 / Chapter 2.1.1 --- Phosphorus is a macronutrient in higher plants --- p.3 / Chapter 2.1.2 --- The forms of phosphorus in plant cells --- p.3 / Chapter 2.1.3 --- Phosphorus compartments and pools in plant cells --- p.6 / Chapter 2.2 --- The acquisition of phosphorus in higher plants --- p.8 / Chapter 2.2.1 --- The forms of phosphorus absorbed by higher plants --- p.8 / Chapter 2.2.2 --- Soil phosphorus bioavailability --- p.9 / Chapter 2.2.3 --- Uptake and transportation of phosphorus --- p.10 / Chapter 2.3 --- Adaptive responses of higher plants to phosphorus deficiency --- p.11 / Chapter 2.3.1 --- Phosphorus homeostasis --- p.12 / Chapter 2.3.2 --- Enhancement of phosphorus uptake --- p.14 / Chapter 2.3.3 --- Phosphorus scavenging and recycling --- p.16 / Chapter 2.4 --- Regulation of gene expression under phosphorus starvation --- p.18 / Chapter 2.5 --- Acid phosphatase and purple acid phosphatase in plants --- p.22 / Chapter 2.5.1 --- Acid phosphatases --- p.22 / Chapter 2.5.2 --- Purple acid phosphatase (PAP) --- p.26 / Chapter Chapter 3: --- Hypothesis --- p.31 / Chapter Chapter 4: --- Materials and Methods --- p.33 / Chapter 4.1 --- Materials --- p.33 / Chapter 4.1.1 --- Chemicals --- p.33 / Chapter 4.1.2 --- Plant materials --- p.33 / Chapter 4.1.3 --- Plasmid vectors and bacterial strains --- p.33 / Chapter 4.1.4 --- DNA sequencing --- p.34 / Chapter 4.1.5 --- Softwares: --- p.34 / Chapter 4.2 --- Methods: --- p.35 / Chapter 4.2.1 --- Survey of PAP occurrence in higher plants --- p.35 / Chapter 4.2.2 --- Determination of multi-gene family and gene copy number of PAPin tomato genome --- p.40 / Chapter 4.2.3 --- Effect of environmental Pi on the morphology of tomato and APase induction --- p.43 / Chapter 4.2.4 --- PAP expression in tomato seedlings growing at different Pi concentrations --- p.46 / Chapter 4.2.5 --- Genomic library construction and PAP promoter isolation --- p.48 / Chapter 4.2.6 --- PAP promoter activity assay by transient expression of reporter gene..… --- p.52 / Chapter Chapter 5: --- Results --- p.56 / Chapter 5.1 --- Identification of PAP gene in higher plants --- p.56 / Chapter 5.1.1 --- Design of primers and total RNA extraction --- p.56 / Chapter 5.1.2 --- RT-PCR --- p.57 / Chapter 5.1.3 --- Further investigation of PAP homologous sequences in monocotyledons --- p.60 / Chapter 5.2 --- Determination of multi-gene family and gene copy number of tomato PAP gene (TPAP 1) --- p.62 / Chapter 5.2.1 --- Determination of TPAP 1 copy number --- p.62 / Chapter 5.2.2 --- Determination of tomato PAP multi-gene family --- p.63 / Chapter 5.3 --- Effect of environmental phosphorus on the morphology of tomato seedling and APase induction --- p.66 / Chapter 5.3.1 --- Morphological changes of tomato seedlings under phosphorus starvation --- p.66 / Chapter 5.3.2 --- Acid phosphatase assays --- p.72 / Chapter 5.4 --- The phosphorus-regulated expression of tomato PAP --- Northern blot analysis --- p.74 / Chapter 5.5 --- Genomic library construction and PAP promoter isolation --- p.76 / Chapter 5.6 --- PAP promoter sequence --- p.79 / Chapter 5.7 --- Promoter activity assay through transient expression of reporter gene --- p.84 / Chapter 5.7.1 --- Effect of untranslation region of PAP gene --- p.84 / Chapter 5.7.2 --- Assay of PAP promoter activities regulated by phosphorus --- p.85 / Chapter Chapter 6: --- Discussion --- p.88 / Chapter 6.1 --- The wide occurrence and high conservation of plant PAP --- p.88 / Chapter 6.2 --- Tomato as a model plant and the organization of PAP gene in genome --- p.89 / Chapter 6.3 --- Morphological changes of tomato under phosphorus starvation and the induction of APase --- p.90 / Chapter 6.4 --- Regulation of PAP in tomato --- p.92 / Chapter 6.5 --- Isolation of PAP promoter --- p.92 / Chapter 6.6 --- Assay of PAP promoter activity --- p.93 / Chapter 6.7 --- Future perspectives --- p.94 / Chapter Chapter 7: --- Conclusion --- p.95 / References --- p.97

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_323518
Date January 2001
ContributorsZhang, Siyi., Chinese University of Hong Kong Graduate School. Division of Biology.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xv, 109 leaves : ill. ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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