Detection of p16 through immunohistochemistry (IHC) is the standard for determining the
HPV status of the tumor according the TNM eighth edition released in 2017 and has become crucial
for determining the HPV status of oropharyngeal squamous cell carcinomas (OPSCC) with direct
impact on staging and prognostication. In recent years, detection of HPV DNA in mouthwashes
has been proposed as a noninvasive alternative, both for OPSCCs and for other head and neck
squamous cell carcinomas (HNSCCs). However, the prospect of using the mouthwashes to monitor
the response to therapy is unclear. To evaluate the effect of curative therapy on the detection of HPV
DNA, we performed a prospective study comparing the detection frequency of high-risk HPV DNA
(HR-HPV-DNA) in pre- and post-therapy mouthwashes. We collected 137 mouthwashes from 88
pathologically confirmed HNSCC patients for DNA isolation and HPV genotyping with the Inno-
LiPA assay. We show that HPV DNA in pretherapeutic mouthwashes can detect HPV-driven HNSCCs
with a sensitivity of 50.0% and specificity of 85.4%, alongside a high negative predictive value of
79.5% and an accuracy of 74.5%. Furthermore, we observed a notable decrease in the detection
frequency of HR-HPV-DNA after successful treatment (pre-therapy 50.0% (9/18) versus post-therapy
9.7% (3/28)). However, the comparatively low sensitivity regarding detection of HPV-driven OPSCC argues against its use in clinical routine.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:90349 |
| Date | 06 March 2024 |
| Creators | Loermann, Gera, Kolb, Marlen, Prascevic, Dusan, Siemert, Julia, Wiegand, Susanne, Zebralla, Veit, Pirlich, Markus, Stöhr, Matthäus, Dietz, Andreas, Wald, Theresa, Wichmann, Gunnar |
| Publisher | MDPI |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 2077-0383, 10.3390/jcm11195509 |
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