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Previous issue date: 2011-11-18 / The tumor hypoxia modulates a series of genetic changes related to adaptive development, invasion and metastasis of various human cancers, among which squamous cell carcinoma of the tongue (SCCT). The objective of this study was to analyze clinical, morphological and immunohistochemical expression by HIF-1α, GLUT-1 and CA-IX in 57 cases of CEL and correlated this expression to clinical parameters and morphological. After a descriptive analysis of data on gender, age, race, and habits of patients, it was found that the results were consistent with the literature. The clinical and morphological parameters analyzed and the expression of these markers of hypoxia were subjected to statistical analysis (Qui2 test), verifying that they can be used as indicators of the biological behavior of CEL. Among the results of this study, we observed that the intensity of expression for HIF-1α, in most cases located in the cytoplasm and nucleus, statistically correlated with clinical staging (p = 0.011) and histological grading (p = 0.002). As for the relationship between the distribution of labeling for HIF-1α and metastasis, the chi-square (Qui2) showed that there was statistically significant differences between the groups (p = 0.040). 75.8% of the sample who had metastases, there was the predominance of diffuse marking. The immunoexpression cytoplasmic/membrane GLUT-1 showed a statistically significant correlation with the clinical stage (p = 0.002) and histological grading (p = 0.000). Concerning the location of markings for GLUT-1 tumor on the island, there was a predominance of peripheral marking specimens in most low-grade (78.6%). In the sample of high-grade, prevailed the location center/periphery (55.8%). According to the chi-square (Qui2), the location on the island of the tumor (p = 0.025) showed statistically significant difference in histological grading. The immunoreactivity of CA-IX, in most cases located in the membrane and cytoplasm, exhibited a statistically significant correlation with histological grading (p = 0.005). Based on these results, we can conclude a broad participation of these markers of hypoxia in oral carcinogenesis and its possible use as markers of biological behavior and tumor progression in CEL / A hip?xia tumoral modula uma s?rie de mudan?as gen?ticas adaptativas relacionadas ao desenvolvimento, invas?o e met?stase de diversos c?nceres humanos, dentre os quais o carcinoma epiderm?ide de l?ngua (CEL). O objetivo do presente trabalho foi realizar uma an?lise cl?nica, morfol?gica e imuno-histoqu?mica atrav?s da express?o do HIF-1α, GLUT-1 e da CA-IX em 57 casos de CEL, correlacionando essa express?o ? par?metros cl?nicos e morfol?gicos. Ap?s uma an?lise descritiva dos dados referentes ao sexo, faixa et?ria, ra?a e h?bitos dos pacientes, constatou-se que os resultados encontrados foram condizentes com a literatura. Os par?metros cl?nicos e morfol?gicos analisados e a express?o desses marcadores de hip?xia foram submetidos ? an?lise estat?stica (teste do Qui2), verificando-se que os mesmos podem ser utilizados como indicadores do comportamento biol?gico do CEL. Dentre os resultados da presente pesquisa, observou-se que a intensidade de express?o para o HIF-1α, localizada na maioria dos casos no citoplasma e n?cleo, correlacionou-se estatisticamente com o estadiamento cl?nico (p = 0,011) e grada??o histol?gica (p = 0,002). Quanto ? rela??o entre a distribui??o de marca??o para o HIF-1α e met?stase, o teste qui-quadrado (Qui2) demonstrou haver diferen?as estatisticamente significativas entre os grupos analisados (p = 0,040). Dos 75,8% da amostra que tinham met?stase, constatou-se a o predom?nio da marca??o difusa. A imunoexpress?o citoplasm?tica/membranar do GLUT-1 exibiu uma correla??o estatisticamente significativa com o estadiamento cl?nico (p = 0,002) e grada??o histol?gica (p = 0,000). Em rela??o ? localiza??o de marca??o para o GLUT-1 na ilha tumoral, evidenciou-se predom?nio da marca??o perif?rica na maioria dos esp?cimes de baixo grau (78,6%). Na amostra de alto grau, prevaleceu a localiza??o centro/periferia (55,8%). De acordo com o teste qui-quadrado (Qui2), a localiza??o na ilha tumoral (p = 0,025) demonstrou haver diferen?as estatisticamente significativas com a grada??o histol?gica. A imunoexpress?o da CA-IX, localizada na maioria dos casos na membrana e citoplasma, exibiu uma correla??o estatisticamente significativa com a grada??o histol?gica (p = 0,005). Com base nestes resultados, pode-se concluir uma ampla participa??o desses marcadores de hip?xia na carcinog?nese oral, bem como a sua poss?vel utiliza??o como marcadores do comportamento biol?gico e da progress?o tumoral em CEL
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/17151 |
| Date | 18 November 2011 |
| Creators | Vasconcelos, Marcelo Gadelha |
| Contributors | CPF:59539224420, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4793681A0&dataRevisao=null, Silveira, Ericka Janine Dantas da, CPF:02869049420, http://lattes.cnpq.br/2186658404241838, Turatti, Eveline, CPF:15999361869, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4708618J1, Pinto, Le?o Pereira, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787928A2, Lima, Antonio Adilson Soares de, CPF:46598103487, http://lattes.cnpq.br/6772338720614408, Queiroz, L?lia Maria Guedes |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Patologia Oral, UFRN, BR, Odontologia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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