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Previous issue date: 2007-04-30 / Diabetes mellitus has been associated with bone metabolism alterations, such as osteopenia and osteporosis. So, the search of new anabolic agents promote bone mass gain can be important to prevent osteoporosis. The aim of this study was evaluate zinc anabolic effect over bone in diabetic and post-menopausal osteopenic models. Diabetes was induced by STZ (45mg/Kg of body weight) administration and post-menopausal by bilateral ovariectomy. Adults female Wistar rats (n=65) were divided in 5 groups: control group (n=15), ovariectomized without (n=15) and with zinc supplementation (n=10) groups, diabetic and ovarioctomized without (n=15) and with zinc supplementation (n=10) groups. Studied periods had been untill 90 days. Diabetic condition was confirmed hiperglicemic state and alterations of state with polyuria, polyphagia, polydipsia and glucosuria. Histomorphometric analysis showed that zinc supplementation increased trabecular thickness and reduced trabecular distance significantly in diabetic groups with similar values to those showed in control group. Correlation analysis of histomorphometric parameters with serum glucose concentration showed that more time in hyperglycemia more bone damage, as well as, zinc supplementation contributed to prevent this damage. Elevated serum glucose caused hyperzincuria, phosphaturia and calciuria. Zinc supplementation promoted increased levels of calcium and phosphorous ions in 90th days diabetic group. No alteration was observed by ovariectomy in mineral (Ca, P and Zn) serum and urine concentrations. Total serum Alkaline Phosphatase activity increased in diabetic groups, supplemented or not, compared with control group. However, Tartarate-Resistant Acid Phosphatase, magnesium and serum zinc did not altered in studied groups. Serum albumin was reduced only in diabetic groups. Serum creatinine was unaltered. These results support the hypotesis that zinc can be used to prevent and treat diabetic and post-menopausal osteopenia / Diabetes mellitus est? associado com altera??es do metabolismo ?sseo (osteopenia e/ou osteoporose). Identificar agentes anab?licos que promovam o ganho de massa ?ssea ? de fundamental import?ncia. Assim, o presente trabalho tem por objetivo avaliar o efeito anab?lico do zinco sobre o tecido ?sseo em modelo de osteopenia diab?tica e p?s-menopausa. O diabetes foi induzido pela administra??o de STZ (45 mg/Kg de peso corp?reo) e a p?s-menopausa pela ooforectomia bilateral. Ratas Wistar adultas (n= 65) foram divididas em 5 grupos: grupo controle (n=15), grupo ooforectomizado sem (n =15) e com zinco suplementar (n=10), grupo diab?tico e ooforectomizado sem (n=15) e com zinco suplementar (n=10). O per?odo de estudo foi de at? 90 dias. A condi??o diab?tica foi evidenciada pela hiperglicemia e pelo quadro metab?lico caracter?stico de poli?ria, polifagia, polidpsia e glicos?ria. As an?lises histomorfom?tricas mostraram que a suplementa??o com zinco promoveu um aumento da espessura e diminuiu a dist?ncia trabecular ?ssea significativamente nos grupos diab?ticos para valores pr?ximos aos dos controles e que o volume ?sseo aumentou significativamente, tanto na defici?ncia de estr?geno como nos animais com diabetes ao longo do estudo. A an?lise de correla??o entre par?metros histomorfom?tricos e glicemia evidenciaram que quanto maior o tempo de hiperglicemia maior a a??o delet?ria sobre o tecido ?sseo e que a suplementa??o com zinco minimizou esta a??o com um ganho significativo da massa ?ssea. Os grupos diab?ticos apresentaram hiperzinc?ria, fosfat?ria e calci?ria. A suplementa??o com zinco promoveu um aumento na concentra??o dos ?ons c?lcio e f?sforo s?rico para o grupo diab?tico em 90 dias. A ooforectomia n?o promoveu altera??o nas concentra??es s?ricas e urin?rias dos minerais Ca, P e Zn. A atividade da fosfatase alcalina s?rica total aumentou somente para os grupos diab?ticos, independente da suplementa??o. J? a fosfatase ?cida tartarato-resistente, o magn?sio e o zinco s?rico n?o alteraram para os grupos estudados. Apenas os grupos diab?ticos apresentaram diminui??o da albumina s?rica, embora a creatinina s?rica tenha se apresentado dentro dos valores de refer?ncia. O conjunto de resultados obtidos suportam que zinco poder? ser utilizado na preven??o e tratamento da osteopenia diab?tica e p?s-menopausa
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/13450 |
| Date | 30 April 2007 |
| Creators | Ferreira, Elaine Cristina da Silva |
| Contributors | CPF:05410395808, http://lattes.cnpq.br/4245215108740331, Almeida, Maria das Gra?as, CPF:20054548420, http://lattes.cnpq.br/0321740024191482, Hirata, Mario Hiroyuki, CPF:87969203868, http://lattes.cnpq.br/8551642748793896, Schwarzschild, L?cia de F?tima Campos Pedrosa, CPF:17547644449, http://lattes.cnpq.br/1863589790139155, Rezende, Adriana Augusto de |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Ci?ncias Farmac?uticas, UFRN, BR, Bioan?lises e Medicamentos |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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