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000737488.pdf: 765065 bytes, checksum: 2838b9b15f0909a0ee3d2a23a324a1dd (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / The objective of this research was to determine the occurrence of gastrointestinal parasites and molecularly characterize the species of Cryptosporidium spp. in stool samples from lambs under one year old in the city of Alambari, São Paulo. A total of 193 samples were collected in 126 and the same was done counting eggs per gram of feces (EPG) Gordon and Whitlock (1939), to determine the presence of helminths and coccidia of the genus Eimeria. All samples were evaluated by nested-PCR amplification of DNA fragments subunit 18S rRNA gene for Cryptosporidium. By using the Chi- square and/or Fisher was significant association of the presence of intestinal parasites in relation to gender, race and age of the animals. Of the 126 samples analyzed, 71.4% were positive by OPG, identifying 58.9% of eggs of Strongyles, 5.6% of oocysts of Eimeria spp.; Coinfection in 14.4% of Strongyles and Moniezia spp., and 15.5% Strongyles and Eimeria spp., and 5.6% infection by the three parasites. Stool cultures were positive in 16 samples, with observation of genres: 31.3% Haemonchus spp., 12.5% Trichostrongylus spp.; Oesophagsotumum spp. in one, and 50% mixed infection with Haemonchus and Trichostrongylus. To Eimeria spp., the incidence was 19% of the animals, identifying the species: E. crandallis; E. faurei; E. marsica; E. ovinoidalis; E. pallida; E. parva and E. weybridgensis. The nPCR was positive in 15% and sequencing was performed in 19 samples with detection of species: C. xiaoi in 15 samples, C. ubiquituim three, and C. meleagridis in a sample, the latter being considered zoonotic species / FAPESP: 10/52542-3
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.unesp.br:11449/94677 |
| Date | 26 April 2013 |
| Creators | Zucatto, Anaíza Simão [UNESP] |
| Contributors | Universidade Estadual Paulista (UNESP), Bresciani, Katia Denise Saraiva [UNESP] |
| Publisher | Universidade Estadual Paulista (UNESP) |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | 56 f. : il. |
| Source | Aleph, reponame:Repositório Institucional da UNESP, instname:Universidade Estadual Paulista, instacron:UNESP |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -1, -1 |
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