<p> Temperature-sensitive mutants of the bacteriophage PBS 2 were isolated from lysates treated with various mutagens. Complementation tests assigned the mutants to 10 cistrons. The mutants were mapped by two factor crosses and formed a linear map approximately 50 recombination percent in length. </p> <p> A method for phage transformation was developed. By the use of wild type DNA fragments fractionated according to their guanine + cytosine content in HgCs₂SO₄ gradients, it was possible to determine the base composition of certain regions of the chromosomes. </p> / Thesis / Doctor of Philosophy (PhD)
Identifer | oai:union.ndltd.org:mcmaster.ca/oai:macsphere.mcmaster.ca:11375/17968 |
Date | 11 1900 |
Creators | Herrington, Muriel Bella |
Contributors | Takahashi, I., Biology |
Source Sets | McMaster University |
Language | English |
Detected Language | English |
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