Primordial gonocytes (PGCs) in some animals, including fish arise after fertilization in extragonadal region from maternally inherited germline cytoplasm, and migrate to the future gonads region during embryogenesis, where differentiate into gametes. PGCs formation and migration patterns have been studied in several species models, and it is known that these patterns differ from each other. Sturgeons belong to class ray-finned fishes(Actinoptergii), in which the sturgeon phylogenetic position is an out-group to teleost fishes, the sturgeon development pattern is more similar to amphibians than teleost fishes. In this study, we demonstrate an injection technique for sturgeon PGCs visualization by GFP nos1 3'UTR mRNA. We found that the Sterlet(A. ruthenus) PGCs are specified in the vegetative pole of the embryo. Subsequently, we reported the PGCs migration route. The arisen PGCs actively migrated on the yolky cell mass, yolky extension, and after that passively moved to gonadal ridge. This study provides evidence that the PGCsare specified by maternally inherited germplasm, located in the vegetative part of the embryo. Sturgeon PGCs specification was similar to that of anuras, but the migration pattern resembled that of teleost. Furthermore, we successfully isolated PGCs to next needed studies.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:173913 |
| Date | January 2014 |
| Creators | DVOŘÁK, Matěj |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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