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Previous issue date: 2012-01-25 / The search for materials that can replace or restore damaged tissues is due to the search for better life quality of the population. Considering that cardiovascular diseases are the main causes of death in Brazil (Banco de Sa?de, 2010), needs some devices that have efficacy in cardiac regeneration, addressing about issues of long-term safety and promoting the healing of vascular vessels (PR Newswire, 2010). In this sense, this paper developed the synthesis of biostable polyurethanes from the reaction of polycaprolactone diol (PCL) with different diisocyanates (HDI and H12MDI), characterizing them by morphological analysis (MEV), thermal (TGA and DSC), mechanical (DMA) and structural (IV), but also evaluating their in vitro cytotoxicity, by testing for the evaluation of cell viability (MTT and VN test) and cell proliferation (Trypan Blue test) using NIH-3T3 fibroblast cells, comparing them with commercial materials, Dacron and PTFE. The obtained results showed that the synthesized polyurethanes had average molar weight (Mw) ranging between 30000 and 263000 g/mol, as well as a plasticity depended of polyol used. The mechanical behavior of polyurethanes showed similar or greater than the commercial material, Dacron and PTFE. The biostability tests showed that there was mass loss below 20% of the materials exposed to hydrolytic degradation in the period of 270 days and below 5% in the enzymatic degradation in the period of 30 days. The cell viability of polyurethanes was superior to 80%, considered non-cytotoxic. Polyurethanes in this study showed favorable results for its possible use as biomaterial in cardiac applications / A procura por materiais que possam substituir ou restaurar tecidos danificados deve-se ? busca por melhor qualidade de vida da popula??o. Levando em considera??o que doen?as cardiovasculares s?o as principais causas de mortes no Brasil (Banco de Sa?de, 2010), necessita-se de dispositivos que possuam efic?cia na regenera??o card?aca, abordando quest?es de seguran?a ? longo prazo e promovendo a cicatriza??o de vasos vasculares (PR Newswire, 2010). Neste sentido, o presente trabalho desenvolveu a s?ntese de poliuretanos bioest?veis a partir da rea??o de policaprolactona diol (PCL) com diferentes diisocianatos (HDI e H12MDI), caracterizando-os por meio de an?lise morfol?gica (MEV), t?rmica (TGA e DSC), mec?nica (DMA) e estrutural (IV), como tamb?m, avaliando sua citotoxicidade in vitro, atrav?s de testes para a avalia??o da viabilidade celular (teste VN e MTT) e prolifera??o celular (teste Azul de Tripan) utilizando c?lulas fibrobl?sticas NIH-3T3, comparando-os com materiais comerciais, Dacron e PTFE. Os resultados obtidos mostraram que os poliuretanos sintetizados apresentaram massa molar ponderal m?dia (Mw) variando entre 30000 e 263000 g/mol, bem como, uma plasticidade dependente do poliol utilizado. O comportamento mec?nico destes poliuretanos mostrou-se semelhante ou superior aos materiais comerciais, Dacron e PTFE. Os testes de bioestabilidade mostraram que houve perda de massa abaixo de 20% dos materiais expostos ? degrada??o hidrol?tica no periodo de 270 dias e abaixo de 5% na degrada??o enzim?tica no periodo de 30 dias. A viabilidade celular dos poliuretanos mostrou-se superior a 80%, consideradas n?o citot?xicas. Os poliuretanos avaliados neste estudo apresentaram resultados favor?veis para seu uso como biomaterial em poss?veis aplica??es cardiol?gicas
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/3193 |
| Date | 25 January 2012 |
| Creators | Cabral, Emanuelli Louren?o |
| Contributors | Ligabue, Rosane Ang?lica |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Engenharia e Tecnologia de Materiais, PUCRS, BR, Faculdade de Engenharia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -7432719344215120122, 500, 600, -655770572761439785 |
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