Thesis (MSc)--Stellenbosch University, 2012. / ENGLISH ABSTRACT: Almost all processes during the life of a plant are affected by the environment.
Changes in phytohormone, metabolite and protein levels follow in response to
changes in the environment. Plant growth promoting substances can stimulate
changes at these levels to facilitate increased plant growth and yields above what
the plant would normally establish. In this study, the effects of two growth promoting
substances, smoke water (SW) derived from bubbling smoke from the burning of
plant material through water, and a synthetic strigolactone analogue, GR24, on plant
growth and architecture, as well as the proteome and metabalome of salt stressed
Nicotiana benthamiana seedlings were investigated. Physiological studies were
conducted to identify the effects of the growth substances on salt stressed seedlings
in a tissue culture system. Under non-stress conditions, SW treatment increased
seedling fresh mass, root length and leaf area. Under salt stress conditions (100
mM and 150 mM NaCl), SW increased fresh mass, root length, leaf number and
lateral root number significantly. Under non-stress conditions, GR24-treated
seedlings showed increased fresh mass, leaf number and area and root length.
When GR24-treated seedlings were placed under salt stress, the seedlings showed
significant increases in fresh mass, leaf number and lateral root number, but only
marginal increases in root length and leaf area. Despite these similarities, slight
differences were observed in the metabolomes and proteomes of smoke water and
GR24-treated seedlings, both with and without the addition of salt stress. Relatively
few of the differentially expressed proteins could be identified with the instruments
available. Changes in the metabolome indicated that photoassimilation and
photosynthesis could be affected in response to smoke water and GR24 treatment.
Our results suggest that smoke water and GR24 both promote growth under salt
stress conditions in seedlings and we furthermore conclude that, although there are
distinct overlaps between treatments, this is accomplished via slightly different
mechanisms. / AFRIKAANSE OPSOMMING: Gedurende ‘n plant se lewe word omtrent alle prosesse deur die omgewing
geaffekteer. Veranderinge in die omgewing word gevolg deur veranderinge in
hormoon, metaboliet en protein vlakke. Plant groei stimulante affekteer hierdie
vlakke om plant groei en -opbrengs na bo normalle vlakke te verhoog. In hierdie
studie word die effek van twee groei stimulante, rook water verkry deur rook van
plant materiaal deur water te borrel en ‘n sintetiese strigolaktoon, GR24, ondersoek
op ‘n morfologiese, metaboliese en ‘n proteomiese vlak in Nicotiana benthamiana
saailinge. ’n Studie is onderneem om die veranderinge as gevolg van die
onderskeie groei stimulante te ondersoek in ‘n weefsel kultuur sisteem. Rook water
het onder normale groei omstandighede vars en droeë massa, blaar aantal asook
wortel en blaar lengte verhoog. Rook water het na sout behandeling (100 en 150
mM NaCl) steeds vars massa, wortel lengte, blaai aantal en laterale wortel aantal
beduidend verhoog in vergelyking met die sout stres kontrole. Behandeling met
GR24 het ook vars massa, wortel lengte, blaar aantal en grootte verhoog en onder
sout stres met GR24 is ‘n beduidende vergroting opgemerk in vars massa, blaar
grootte en laterale wortel aantal. Ongeag van die veranderinge in groei is klein
verskille opgemerk in die metaboliet en protein studies. Net ‘n paar proteine kon
positief geidentifiseer word met die apparaat beskikbaar. Verandering in die
metaboloom wys na veranderinge in fotoassimilasie en fotosintese in reaksie tot rook
water en GR24. Hierdie resultate lei tot die gevolgtrekking dat rook water en GR24
beide groei verbeter in saailing behandel met sout en ook dat alhoewel daar sekere
ooreenkomste is tussen die reaksies as gevolg van die plant groei stimulante, dit wel
geskiet deur geringe verskillende meganismes.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:sun/oai:scholar.sun.ac.za:10019.1/17863 |
| Date | 03 1900 |
| Creators | Steenkamp, Letitia Elizabeth |
| Contributors | Hills, P. N., Stellenbosch University. Faculty of AgriSciences. Dept. of Genetics. Institute for Plant Biotechnology. |
| Publisher | Stellenbosch : Stellenbosch University |
| Source Sets | South African National ETD Portal |
| Language | en_ZA |
| Detected Language | English |
| Type | Thesis |
| Format | 123 p. : ill. |
| Rights | Stellenbosch University |
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