Diabetic gastroenteropathy is a common complication, which develops in patients with
long-term diabetes. The pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide known for its cytoprotective properties and plays an important role in neuronal development,
neuromodulation and neuroprotection. The present study was designed to elucidate, for the first time,
the impact of prolonged hyperglycaemia conditions on a population of PACAP-like immunoreactive
neurons in selected parts of the porcine gastrointestinal tract. The experiment was conducted on
10 juvenile female pigs assigned to two experimental groups: The DM group (pigs with streptozocin-induced diabetes) and the C group (control pigs). Diabetes conditions were induced by a single
intravenous injection of streptozocin. Six weeks after the induction of diabetes, all animals were
euthanised and further collected, and fixed fragments of the stomach, duodenum, jejunum, ileum
and descending colon were processed using the routine double-labelling immunofluorescence technique. Streptozotocin-induced hyperglycaemia caused a significant increase in the population of
PACAP-containing enteric neurons in the porcine stomach, small intestines and descending colon.
The recorded changes may result from the direct toxic effect of hyperglycaemia on the ENS neurons,
oxidative stress or inflammatory conditions accompanying hyperglycaemia and suggest that PACAP
is involved in regulatory processes of the GIT function in the course of diabetes.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:88811 |
| Date | 03 January 2024 |
| Creators | Palus, Katarzyna, Bulc, Michal, Calca, Jaroslaw, Zielonka, Lukasz, Nowicki, Marcin |
| Publisher | MDPI |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 1422-0067, 10.3390/ijms22115727 |
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