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Previous issue date: 2017-08-18 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Angiostrongylus cantonensis and A. costaricensis can accidentally infect humans
causing eosinophilic meningitis and abdominal angiostrongyliasis, respectively. Both
species have several mollusks as intermediate hosts. Diagnosing the infection
requires killing the mollusks, interfering in conservational and population dynamics
studies. This work had the objective to demonstrate possible biological markers in
infected intermediate hosts, as well as to investigate specific factors related to the
parasite-host relationship of mollusks and Angiostrongylus parasites. Mollusks of the
Veronicellidae family were infected with A. cantonensis L1. Mucus and feces from
these infected and uninfected animals were used for differential expression analysis of
proteins using mass spectrometry and the microbiome profile analysis was performed
through the 16S gene sequencing, respectively. LC-MS/MS spectrometry analysis
showed an increase in F-BAR proteins subfamily and a decrease in the elongation
factor of Mycoplasma spp. Microbiome of feces from infected slugs presented a
decrease of the Bacteroidetes phylum. We have also analyzed the microbiome profile
of Biomphalaria glabrata feces infected with A. cantonensis and Schistosoma
mansoni. We could observe a decrease of Vogesella genus. When infected with S.
mansoni, a reduction of Mycoplasma and Nitrospira genera and an increase in
Niabella genus could be demonstrated. In both infections, decrease of Fluviicola
genus and increase of organisms of the Weeksellaceae family were significant. Our
results showed that proteins and microorganisms could be promising biomarkers of A.
cantonensis infection in intermediate hosts in vivo. During the collection of the
mollusks, the invasive species Meghimatium pictum infected with A. costaricensis
was found, associated to a case of abdominal angiostrongyliasis, reported in this
thesis. Parallel to this study, the postures of Limax sp. and Phyllocaulis sp. were
registered to investigate the embryogenesis of these animals. In postures and in
decomposing mollusks, mites of the species Caloglyphus berlesei were also identified
and this association was observed for the first time. / Angiostrongylus cantonensis e A. costaricensis podem infectar o ser humano
acidentalmente causando a meningite eosinof?lica e a angiostrongil?ase abdominal,
respectivamente. Ambos possuem diversas esp?cies de moluscos como hospedeiros
intermedi?rios. O diagn?stico da infec??o requer a morte destes moluscos,
interferindo em estudos de conserva??o e din?mica populacional. Este trabalho teve
como objetivo principal encontrar poss?veis marcadores biol?gicos para a infec??o de
hospedeiros intermedi?rios, bem como investigar fatores relacionados a rela??o
parasito-hospedeiro entre moluscos e Angiostrongylus cantonensis. Exemplares de
Phyllocaulis sp. foram infectados com larva de primeiro est?dio de A. cantonensis. O
muco e fezes destes animais infectados e n?o infectados foram utilizados para an?lise
de express?o diferencial de prote?nas por espectrometria de massas e an?lise do perfil
do microbioma por sequenciamento do gene ribossomal 16S, respectivamente. As
an?lises de espectrometria por LC-MS/MS mostraram um aumento das prote?nas da
subfam?lia F-BAR e diminui??o do fator de alongamento de Mycoplasma spp. O
microbioma das fezes de lesmas infectadas apresentou uma diminui??o do filo
Bacteroidetes. Tamb?m foram analisados o perfil do microbioma de fezes de
Biomphalaria glabrata infectadas com A. cantonensis e Schistosoma mansoni. Nas
fezes de caramujos infectados com A. cantonensis foram observados uma diminui??o
do g?nero Vogesella; J? quando infectados com S. mansoni, houve redu??o dos
g?neros Mycoplasma e Nitrospira e aumento de Niabella. Em ambas as infec??es, a
diminui??o de Fluviicola e o aumento de organismos da fam?lia Weeksellaceae foram
significativos. Estes resultados mostram que prote?nas e microorganismos s?o
promissores biomarcadores da infec??o de A. cantonensis em hospedeiros
intermedi?rios, objetivando o desenvolvimento de m?todos diagn?sticos in vivo.
Durante as coletas de moluscos, foi encontrada, pela primeira vez, a esp?cie invasora
Meghimatium pictum infectada com A. costaricensis associada a um caso de
Angiostrongil?ase abdominal, relato que comp?e essa tese. Paralelamente a este
estudo, foram acompanhadas as posturas de Limax sp. e Phyllocaulis sp. durante o
desenvolvimento embrion?rio, com o objetivo de registrar e investigar a
embriog?nese destes hospedeiros. Nas posturas e em moluscos em decomposi??o,
foram identificados ?caros da esp?cie Caloglyphus berlesei, em associa??o observada
pela primeira vez.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/7778 |
| Date | 18 August 2017 |
| Creators | Os?rio, Joana Borges |
| Contributors | Graeff-Teixeira, Carlos |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s Gradua??o em Zoologia, PUCRS, Brasil, Escola de Ci?ncias |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -6482652380601267558, 500, 600, 2075167498588264571 |
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