The purpose of this study was to investigate the ultrastructure
of the membrana limitans interna (internal limiting
membrane, ILM) and to evaluate alterations to the retinal cell
layers after membrane peeling with vital dyes. Twenty-five
patients (25 eyes) who underwent macular hole surgery
were included, whereby 12 indocyanine green (ICG)- and 13
brilliant blue G (BBG)-stained ILM were analyzed using light,
transmission electron and scanning electron microscopy.
Retinal cell fragments on the ILM were identified in both
groups using immunohistochemistry. Comparing ICG- and
BBG-stained membranes, larger cellular fragments were observed
at a higher frequency in the BBG group. Thereby, the
findings indicate that ICG permits an enhanced separation
of the ILM from the underlying retina with less mechanical
destruction. A possible explanation might be seen in the
known photosensitivity of ICG, which induces a stiffening
and shrinkage of the ILM but also generates retinal toxic metabolites
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:80186 |
| Date | 27 July 2022 |
| Creators | Brockmann, Tobias, Steger, Claudia, Westermann, Martin, Nietzsche, Sandor, Königsdörffer, Ekkehart, Strobel, Jürgen, Dawczynski, Jens |
| Publisher | Karger |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 4 |
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