by Hui Yee Han, Ellen. / Thesis (M.Sc.)--Chinese University of Hong Kong, 1994. / Includes bibliographical references (leaves 68-71). / acknowledgements --- p.i / abstract --- p.ii / table of contents --- p.iii-vi / list of figures --- p.vii / LIST OF TABLES --- p.viii / chapter / Chapter chapter i : --- introduction --- p.1 / Chapter chapter ii : --- background knowledge --- p.2 -33 / Chapter 2.1 --- Definition of Free Radical --- p.2 / Chapter 2.2 --- Oxygen Derived Radicals and Their Generation In Vivo --- p.2 -9 / Chapter 2.2.1 --- Production of Singlet Oxygen --- p.4 / Chapter 2.2.2 --- Production of Superoxide Radical (O2-) and Hydrogen Peroxide (H2O2) --- p.4 -8 / Chapter I. --- Endogenous Production --- p.4-7 / Chapter II. --- Exogenous Production --- p.7-8 / Chapter 2.2.3 --- Generation of Hydroxy Radical (OH) through H2O2 and O2- --- p.8 -9 / Chapter 2.3 --- Free Radical Damage and Lipid Peroxidation --- p.9 -13 / Chapter 2.4 --- Lipid Peroxidation and Atherosclerosis --- p.13 -14 / Chapter 2.5 --- Antioxidant --- p.15-21 / Chapter 2.5.1 --- Primary Preventive Antioxidants --- p.15-17 / Chapter 2.5.2 --- Secondary Radical Scavenging Antioxidants --- p.18-21 / Chapter I. --- Lipid Soluble Radical-Scavenging Antioxidants --- p.18-19 / Chapter II. --- Water Soluble Radical-Scavenging Antioxidants --- p.20 -21 / Chapter 2.6 --- Measurement of Oxygen-Derived Radical in Vivo --- p.21 -26 / Chapter 2.7 --- Principle of the TRAP assay --- p.27-33 / Chapter 2.7.1 --- Oxygen Consumption Method --- p.29 -30 / Chapter 2.7.2 --- Chemiluminescence Method --- p.31-33 / Chapter chapter III： --- materials and methods --- p.34 -43 / Chapter 3.1 --- Instrumentation and Materials --- p.34 / Chapter 3.2 --- Method --- p.34-43 / Chapter 3.2.1 --- Establishment of Chemiluminescence Method for Determination of TRAP --- p.34 -36 / Chapter I. --- Preparation of Luminometer --- p.35 / Chapter II. --- Preparation of Sample before Analysis --- p.3 5 / Chapter III. --- Manual Operation of the Chemiluminescence Method --- p.35-36 / Chapter IV. --- Calculation of TRAP --- p.36 / Chapter 3.2.2 --- Evaluation of the Chemiluminescence Method --- p.37 -42 / Chapter I. --- Linearity --- p.37 / Chapter II. --- Recovery --- p.37-38 / Chapter III. --- Precision --- p.39 / Chapter IV. --- Interference Experiment --- p.39 -41 / Chapter V. --- Effect of Storage on TRAP --- p.42 / Chapter 3.2.3 --- "Determination of Analytical, Intraindividual and Interindividual Biological Variation of TRAP in A Group of Healthy Chinese" --- p.42-43 / Chapter CHAPTER IV: --- ANALYTICAL RESULTS --- p.44-56 / Chapter 4.1 --- Method Evaluation --- p.44-51 / Chapter 4.1.1 --- Linearity --- p.44 / Chapter 4.1.2 --- Recovery --- p.45 / Chapter 4.1.3 --- Within-Day and Between-Day Precision --- p.46-47 / Chapter 4.1.4 --- Interference --- p.47-48 / Chapter 4.1.5 --- Effect of Storage on TRAP --- p.48-51 / Chapter 4.2 --- "Analytical, Intraindividual and Interindividual Variation of TRAP in A Group of Healthy Chinese Population" --- p.52 -56 / Chapter 4.2.1 --- Difference in TRAP value obtained from the 22 subjects over time --- p.52 -54 / Chapter 4.2.2 --- The Effect of Genders on Trap --- p.55 / Chapter 4.2.3 --- "Determination of Analytical, Intraindividual and Interindividual Variation of TRAP in A Group of Healthy Chinese" --- p.55-56 / Chapter CHAPTER V: --- DISCUSSION --- p.57-67 / Chapter 5.1 --- Validation of the Method Performance --- p.57 / Chapter 5.2 --- Effect of Storage on TRAP --- p.57 / Chapter 5.3 --- Interference of Hemolysis and Lipemia on TRAP assay --- p.58-60 / Chapter 5.3.1 --- Effect of Hemolysis on TRAP --- p.58-59 / Chapter 5.3.2 --- Effect of Lipemia on TRAP --- p.59-60 / Chapter 5.4 --- Possible Sources of Variation in TRAP Over Time --- p.60 -63 / Chapter 5.5 --- Usefulness of the Variation Data of TRAP obtained from a Group of Healthy Chinese --- p.64 -67 / reference --- p.68 -71
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_318160 |
| Date | January 1994 |
| Contributors | Hui, Yee Han Ellen., Chinese University of Hong Kong Graduate School. Division of Pathological Sciences. |
| Publisher | Chinese University of Hong Kong |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, viii, 71 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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