In West African medicine, Entada africana Guill. & Perr. from the family of Fabaceae is used
to treat inflammatory conditions in the management of fractures, wounds, and sprains in
the northern region of the Republic of Benin. The aim of the present study was to isolate
and elucidate phenolic compounds from a hydroalcoholic leaf extract from E. africana and
to identify compounds with anti-inflammatory activity in vitro. Eleven compounds were
purified from three fractions, which have shown strong to medium anti-inflammatory
activity. The isolated compounds were characterized by HRESI-MS and NMR methods as
gallic acid (1), ethyl gallate (2), 5,7-dihydroxychromen-4-one (3), 3′,4′,7-trihydroxyflavone
(4), dihydrokaempferol-7-O-glucoside (5), catechin (6), quercetin-3-O-[β-apiosyl-
(1‴→2″)-β-glucoside] (7), quercetin-3-O-glucoside (8), naringenin-7-O-glucoside (9),
aromadendrin (10), and myricetin-3-O-glucoside (11). Nine of the major phenolic
compounds were tested using TNF-α stimulated human keratinocytes (HaCaT) as skin
inflammation model to identify molecules, which may explain the use of the plant leaves as
an anti-inflammatory remedy by assessing the release of proinflammatory cytokines IL-8
and IL-6. The hydroacoholic leaf extract of E. africana exerted a medium inhibitory effect on
the release of IL-8. 3′,4′,7-trihydroxyflavone, aromadendrin, dihydrokaempferol-7-
O-glucoside and ethyl gallate demonstrated a strong to medium effect on the release
of IL-6. For the release of IL-8, 3′,4′,7-trihydroxyflavone demonstrated a medium activity.
This study provides for the first time a detailed screening of phenolic compounds occurring
in the hydroethanolic leaf extract of E. africana. Additionally, it is shown that E. africana
contains active compounds which may justify its traditional medicinal use as an antiinflammatory
remedy to treat inflammatory and pain-related skin conditions in the Republic
of Benin.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:87515 |
| Date | 20 October 2023 |
| Creators | Codo Toafode, Nonvignon Murielle, Marquardt, Peter, Ahyi, Virgile, Fester, Karin, Spiegler, Verena, Vissiennon, Cica |
| Publisher | Frontiers Media S.A. |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 931240 |
Page generated in 0.0021 seconds