Many groups of protists, also known as microbial eukaryotes, remain understudied. One such group, the superfamily Sainouroidea in the supergroup Rhizaria has been shown to be a molecularly diverse and divergent clade by recent environmental sequencing. Here we isolated and analyzed cultures of Sainouroid amoebae using 18S rDNA sequencing, light microscopy, and TEM. Using a molecular species concept we described four novel genera and twelve novel species in Sainouroidea. Another understudied group Cutosea, in the supergroup Amoebozoa, contains the ATCC isolate PRA-29, which is an amoeba that has been used in many phylogenomic datasets. PRA-29 was incorrectly identified as Pessonella sp. Here we studied PRA-29 in detail using light microscopy, TEM, and SEM and re-classify it as Armaparvus languidus novel genus, novel species. We show that a cell coat of microscales separated from the cell membrane is a unique trait found in all known Cutosean amoebae.
| Identifer | oai:union.ndltd.org:MSSTATE/oai:scholarsjunction.msstate.edu:td-2065 |
| Date | 10 August 2018 |
| Creators | Schuler, Gabriel |
| Publisher | Scholars Junction |
| Source Sets | Mississippi State University |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | Theses and Dissertations |
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