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Characterization of the Pigment-Protein and Pigment-ester of Xanthomonas Campestris Pv. Juglandis

The objectives of this project were to develop a high performance liquid chromatographic method for separating the pigment esters mixture, to determine the locations of the pigment moiety in the isolated esters using pholosiphases, and to characterize the pigment-protein complex and determine its distribution in other bacteria. Saponification of the two pigment esters 1 and 2 with aqueous KOH yielded two free pigments on TLC plates developed by two solvent systems. The fasters moving of these two free pigments co-chromatographed with the one free pigment produced from each pigment ester by phospholipase A2 treatment. This suggests that the pigment molecule is a methoxy derivative of xanthomonadin and is esterified to the 2-position of the glycerol moiety of each pigment ester. No free pigment was released from phospholipases C and D treatment of the two pigment esters, indicating that pigment is not esterified to the sorbitol or phosphate moiety of pigment esters 1 or 2.

Identiferoai:union.ndltd.org:unt.edu/info:ark/67531/metadc798279
Date05 1900
CreatorsLawani, Leonard Olu
ContributorsKester, Andrew S., Barrow, William W., Eisenberg, Arthur J., O'Donovan, Gerard A., Skinner, Charles Gordon
PublisherNorth Texas State University
Source SetsUniversity of North Texas
LanguageEnglish
Detected LanguageEnglish
TypeThesis or Dissertation
Formatvii, 141 leaves : ill., Text
RightsPublic, Lawani, Leonard Olu., Copyright, Copyright is held by the author, unless otherwise noted. All rights

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