The objectives of this project were to develop a high performance liquid chromatographic method for separating the pigment esters mixture, to determine the locations of the pigment moiety in the isolated esters using pholosiphases, and to characterize the pigment-protein complex and determine its distribution in other bacteria. Saponification of the two pigment esters 1 and 2 with aqueous KOH yielded two free pigments on TLC plates developed by two solvent systems. The fasters moving of these two free pigments co-chromatographed with the one free pigment produced from each pigment ester by phospholipase A2 treatment. This suggests that the pigment molecule is a methoxy derivative of xanthomonadin and is esterified to the 2-position of the glycerol moiety of each pigment ester. No free pigment was released from phospholipases C and D treatment of the two pigment esters, indicating that pigment is not esterified to the sorbitol or phosphate moiety of pigment esters 1 or 2.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc798279 |
| Date | 05 1900 |
| Creators | Lawani, Leonard Olu |
| Contributors | Kester, Andrew S., Barrow, William W., Eisenberg, Arthur J., O'Donovan, Gerard A., Skinner, Charles Gordon |
| Publisher | North Texas State University |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | vii, 141 leaves : ill., Text |
| Rights | Public, Lawani, Leonard Olu., Copyright, Copyright is held by the author, unless otherwise noted. All rights |
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