La manzana y la uva son frutas muy consumidas por el hombre, especialmente en nuestro medio ya que forma parte de la lonchera, especialmente de los niños, por ello se investigó la presencia de plaguicidas órgano fosforados por el método de la AOAC Oficcial Method 2007.01 “Pesticide Residues in Foods by Acetonitrile Extraction and Partioning with Magnesium Sulfate”. La extracción se hizo con acetonitrilo en medio tamponado con ácido acético-acetato de sodio seguido de un clean-up combinada con amina primaria secundaria y sulfato de magnesio, para finalmente ser analizado por LC/MS-MS (cromatógrafo líquido acoplada con espectrofotómetro de masas en tándem y GC-MS (cromatógrafo de gases acoplada a espectrofotómetro de masas). Se tomaron 10 muestras de manzanas (05 de Calango y 05 de Santa Cruz de Flores) y 10 muestras de uvas (05 muestras de Calango y 05 muestras de Santa Cruz de Flores) de la provincia de Cañete. No se encontraron residuos de plaguicidas órgano fosforados. El 100 por ciento de las uvas presentan residuos de otros plaguicidas tales como Ciprodenil y Piroclostrobin y el 50 por ciento de las manzanas presentan los plaguicidas Tebuconazol y Cipermetrina. Los agricultores de los distritos de Calango y Santa Cruz de Flores no reciben información sobre el peligro del uso de los plaguicidas y no utilizan los implementos de protección personal adecuada. Palabras clave: LC/MS-MS, GC/MS, residuo, plaguicida, clean-up. / --- Apples and grapes are fruits very consumed by people, especially in our country as part of the lunchbox, particularly for children. For that reason, it was investigated the presence of phosphorus pesticides bodies by the AOAC Official Method 2007.01 - "Pesticide Residues in Foods by Acetonitrile Extraction and partitioning with Magnesium Sulfate". The extraction was done with acetonitrile in buffered medium with acetic acid - sodium acetate followed by a clean-up, combined with primary secondary amine, and magnesium sulfate, to finally be analyzed by LC / MS-MS (liquid chromatograph accepted with mass spectrometer in Tandem) and GC-MS (gas chromatograph coupled to mass spectrometer). Ten (10) samples of apples, five (5) samples of Calango and five (5) samples of Santa Cruz de Flores, from the Cañete province were taken. No organ-phosphorus pesticide residues were found. The 100% of the grapes presents other pesticides residues such as and Pyraclostrobin and Cyprodinil, and the 50% of apples presents pesticides such as tebuconazole and cypermethrin. Calango and Santa Cruz de Flores districts do not receive information about the danger of the use of pesticides and do not use the proper personal protective equipment. Keywords: LC/MSMS, GC-MS, Residue, Pesticide, Clean-Up.
| Identifer | oai:union.ndltd.org:Cybertesis/oai:cybertesis.unmsm.edu.pe:cybertesis/4651 |
| Date | January 2016 |
| Creators | Lizano Gutierrez, Jesús Victor |
| Contributors | Nancy Lozano Reyes |
| Publisher | Universidad Nacional Mayor de San Marcos |
| Source Sets | Universidad Nacional Mayor de San Marcos - SISBIB PERU |
| Language | Spanish |
| Detected Language | Spanish |
| Type | info:eu-repo/semantics/masterThesis |
| Source | Repositorio de Tesis - UNMSM, Universidad Nacional Mayor de San Marcos |
| Rights | info:eu-repo/semantics/openAccess |
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