Leung, Ho Wai. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2007. / Includes bibliographical references (leaves 136-146). / Abstracts in English and Chinese. / THESIS COMMITTEE --- p.II / ACKNOWLEDGEMENT --- p.III / ABSTRACT --- p.IV / CHINESE ABSTRACT --- p.VI / TABLE OF CONTENT --- p.VII / OVERVIEW OF THIS PROJECT --- p.1 / Chapter SECTION 1: --- Purification and Characterization of hemagglutinins from French bean and mottled kidney bean / Chapter Chapter 1 --- INTRODUCTION / Chapter 1.1 --- General Introduction --- p.2 / Chapter 1.2 --- Physiological functions of plant lectins --- p.6 / Chapter 1.3 --- Physiological functions of animal lectins --- p.9 / Chapter 1.4 --- Biological functions of lectins --- p.12 / Chapter 1.5 --- Clinical and research applications of lectins --- p.16 / Chapter 1.6 --- Legume lectins --- p.17 / Chapter 1.7 --- Isolation and purification of lectins --- p.19 / Chapter 1.8 --- Objectives of the present study --- p.21 / Chapter Chapter 2 --- MATERIALS AND METHODS / Chapter 2.1 --- Chemicals --- p.22 / Chapter 2.2 --- Assay of hemagglutinating activity --- p.24 / Chapter 2.3 --- Purification protocol --- p.26 / Chapter 2.4 --- Assay of saccharide inhibition of hemagglutination --- p.28 / Chapter 2.5 --- Assay of pH stability --- p.28 / Chapter 2.6 --- Molecular mass determination and N-terminal sequence determination --- p.28 / Chapter 2.7 --- Assay of mitogenic activity --- p.29 / Chapter 2.8 --- Assay of antiproliferative activity --- p.30 / Chapter 2.9 --- Assay for antifungal activity --- p.30 / Chapter 2.10 --- Assay of HIV-1 reverse transcriptase inhibitory activity --- p.31 / Chapter 2.11 --- Assay of stability towards trypsin and chymotrypsin --- p.31 / Chapter 2.12 --- Assay of nitric oxide production --- p.32 / Chapter 2.13 --- Assay ofHIV-1 integrase --- p.32 / Chapter Chapter 3 --- EXPERIMENTAL RESULTS / Chapter 3.1 --- Purification scheme --- p.35 / Chapter 3.2 --- Size determination and N-terminal sequencing --- p.36 / Chapter 3.3 --- Temperature stability assay --- p.37 / Chapter 3.4 --- pH stability assay --- p.37 / Chapter 3.5 --- Saccharides inhibition of hemagglutination --- p.37 / Chapter 3.6 --- Stability towards Trypsin and Chymotrypsin --- p.38 / Chapter 3.7 --- Anti-proliferative activity --- p.38 / Chapter 3.8 --- HTV-1 reverse transcriptase inhibition --- p.39 / Chapter 3.9 --- Mitogenic activity --- p.39 / Chapter 3.10 --- Nitric oxide production --- p.39 / Chapter 3.11 --- HIV-1 integrase --- p.39 / Chapter 3.12 --- Defensin --- p.40 / Chapter Chapter 4 --- DISCUSSION / Chapter 4.1 --- Purification scheme --- p.68 / Chapter 4.2 --- Sequence comparison --- p.69 / Chapter 4.3 --- Physical Stability of the hemagglutinins --- p.70 / Chapter 4.4 --- Protease Stability --- p.71 / Chapter 4.5 --- Sugar Specificity Assay --- p.72 / Chapter 4.6 --- Anti-proliferative Aactivity toward Cancer Cells --- p.73 / Chapter 4.7 --- HTV-1 reverse trancriptase and H̐ơþV integrase inhibition --- p.74 / Chapter 4.8 --- Mitogenic activity --- p.75 / Chapter 4.9 --- Antifungal protein --- p.76 / Chapter Chapter 5 --- CONCLUSION --- p.78 / Chapter SECTION 2: --- Purification and Characterization of flammulolysin from mushroom Flαmmulinα velutipes / Chapter Chapter 1 --- INTRODUCTION / Chapter 1.1 --- General Introduction --- p.79 / Chapter 1.2 --- Mechanisms of hemolysis --- p.80 / Chapter 1.3 --- Biological role of hemolysins --- p.80 / Chapter 1.4 --- Mushroom hemolysin --- p.82 / Chapter 1.5 --- Applications of hemolysins --- p.83 / Chapter 1.6 --- Objectives of the present study --- p.83 / Chapter Chapter 2 --- MATERIALS AND METHODS --- p.84 / Chapter Chapter 3 --- EXPERIMENTAL RESULTS / Chapter 3.1 --- Purification and sequence determination --- p.90 / Chapter 3.2 --- Effect of sugars and salts on hemolysin --- p.90 / Chapter 3.3 --- Effect of Temperature and pH on hemolysin --- p.91 / Chapter 3.4 --- Effect of Proteases on hemolysin --- p.91 / Chapter 3.5 --- Effect of osmotic protection on hemolysin --- p.91 / Chapter 3.6 --- Effect of hemolysin on tumor cells --- p.91 / Chapter 3.7 --- Effect of hemolysin on spleen cells --- p.92 / Chapter 3.8 --- Effect of hemolysin on bacterial growth --- p.92 / Chapter 3.9 --- Effect of hemolysin on fungal growth --- p.92 / Chapter Chapter 4 --- DISCUSSION / Chapter 4.1 --- Purification and sequence comparison of hemolysin --- p.103 / Chapter 4.2 --- Sugar and Salts inhibition --- p.104 / Chapter 4.3 --- Temperature stability --- p.105 / Chapter 4.4 --- pH stability --- p.106 / Chapter 4.5 --- Protease stability --- p.106 / Chapter 4.6 --- Osmotic Protection --- p.106 / Chapter 4.7 --- Anti-tumour activity of the hemolysin --- p.107 / Chapter 4.8 --- Anti-fungal activity --- p.108 / Chapter Chapter 5 --- CONCLUSION --- p.109 / Chapter SECTION 3: --- Purification and Characterization of antifungal peptide from buckwheat seeds Fagopyrum esculentum / Chapter Chapter 1 --- INTRODUCTION / Chapter 1.1 --- Plant antiftmgal proteins --- p.110 / Chapter 1.2 --- Classification of antifungal proteins --- p.110 / Chapter 1.3 --- Distribution of antifungal proteins in plants --- p.111 / Chapter 1.4 --- Mechanisms of antifungal activity --- p.111 / Chapter 1.5 --- Future Perspectives of Antifungal proteins --- p.112 / Chapter 1.6 --- Antifungal peptide from Buckwheat --- p.112 / Chapter 1 .7 --- Objectives of the present study --- p.113 / Chapter Chapter 2 --- MATERIALS AND METHODS --- p.114 / Chapter Chapter 3 --- EXPERIMENTAL RESULTS / Chapter 3.1 --- Purification and sequence determination --- p.118 / Chapter 3.2 --- Effect on anti-fungal activity --- p.118 / Chapter 3.3 --- Effect of temperature and pH on antifungal activity --- p.118 / Chapter 3.4 --- Effect of the antifungal peptide on tumor cells --- p.119 / Chapter 3.5 --- Effect of antifungal peptide on HIV-1 Reverse transcriptase Activity --- p.119 / Chapter 3.6 --- Effect of antifungal peptide on spleen cells and NO Production --- p.119 / Chapter Chapter 4 --- DISCUSSION / Chapter 4.1 --- Purification scheme and N-terminal sequence --- p.130 / Chapter 4.2 --- Antifungal Activity --- p.131 / Chapter 4.3 --- Physical stability --- p.131 / Chapter 4.4 --- Anti-proliferative activity toward cancer cells --- p.131 / Chapter 4.5 --- HTV-1 Reverse Transcriptase Inhibitory activity --- p.132 / Chapter 4.6 --- Mitogenic activity and nitric oxide production --- p.132 / Chapter Chapter 5 --- CONCLUSION --- p.133 / OVERALL CONCLUSION --- p.134 / REFERENCES --- p.136
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_326033 |
| Date | January 2007 |
| Contributors | Leung, Ho Wai., Chinese University of Hong Kong Graduate School. Division of Biochemistry. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, x, 146 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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